天津医药

• 实验研究 •    

热休克预处理对大鼠胰腺移植后缺血性再灌注损伤的抑制作用

裴广辉1,宋文利2,梁健3,王智平2,莫春柏2   

  1. 1. 天津市第一中心医院移植外科A3
    2. 天津市第一中心医院移植外科
    3. 中国医科大学附属第四医院肝胆胰外科
  • 收稿日期:2010-03-17 修回日期:2010-06-13 出版日期:2010-08-15 发布日期:2010-08-15
  • 通讯作者: 梁健

Heat Shock Preconditioning on Pancreatic Graft Reduces Posttransplant Ischemia Reperfusion Injury of Rat

  • Received:2010-03-17 Revised:2010-06-13 Published:2010-08-15 Online:2010-08-15

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摘要： 摘要 目的：通过热休克预处理（HPC），探讨减轻大鼠胰腺移植后缺血再灌注损伤的发生机制。 方法：热休克预处理后动态检测大鼠胰腺组织热休克蛋白表达。建立大鼠胰腺移植缺血再灌注模型，选择表达热休克蛋白高峰时段供体大鼠胰腺移植作为实验组，未预处理的供体大鼠胰腺移植作为对照组。胰腺移植后6h，检测静脉血及移植胰腺。Western blot法检测胰腺组织中热休克蛋白70（HSP70）的表达，免疫组化法测定肿瘤坏死因子α（TNF-α）表达。碘淀粉比色法检测血淀粉酶水平。光镜观察胰腺病理改变。 结果：HPC后供体大鼠胰腺中HSP70的表达在12h 达到高峰，与其它各时段比较具有显著差异（P<0.05），与未预处理组比较差异也显著（P<0.01），48h恢复到原来水平。对照组胰腺组织中TNF-α、中性粒细胞、血淀粉酶的水平明显升高，与假手术组相比差异显著（P<0.01）。实验组降低了胰腺组织中TNF-α、中性粒细胞、血淀粉酶的水平，与对照组比较差异具有统计学意义。（P<0.05）。实验组胰腺间质水肿及出血明显低于对照组。 结论：热休克预处理可以减轻大鼠胰腺移植后缺血再灌注损伤, 热休克预处理的延迟保护作用与HSP70的诱导生成有关。

关键词: 热休克预处理, 胰腺移植, 缺血再灌注损伤, 热休克蛋白70

Abstract: Abstract Objective：Rats were heat shock preconditioned to determine the mechanism that heat shock precondition(HPC) reduce ischemia reperfusion injury of rats model after pancreas transplantation. Method：Expression of heat shock protein70 (HSP70)of pancreas of rats who were heat shock preconditioned was detected at different time. rats model of ischemia reperfusion injury after pancreas transplantation were set up. Donator rats of high expression of HSP70 were regarded as experiment group for pancreas transplantation and donator rats of non- heat shock precondition as control group.Blood and pancreas of rats were examined after pancreas transplantation after 6 hours.Expression of HSP70 in pancreas was examined with western-blotting. Expression of TNF-α in pancreas was detected with immunohistochemistry. Hemodiastase of rats was determined with iodoamylum chromatometry.Pathological changes of pancreas was detected with microscope. Result：Expression of HSP70 of pancreas of donator rats achieves peak after HPC 12 hours, which has significant difference comparison with other time points （P<0.05）, and which has significant difference comparison with no precondition group（P<0.01）,recovery after HPC 48 hours. Levels of TNF-α、neutrophil and hemodiastase of pancreatic tissue significantly increased in control group comprared with that in sham group（P<0.01）。Levels of TNF-α、neutrophil and hemodiastase of pancreatic tissue significantly decreased in experiment group comprared with that in control group（P<0.05）,and interstitial edema and hemorrhage of pancreas were reduced in experiment group. Conclusion：Heat shock preconditioning reduce ischemia reperfusion injury of rats model after pancreas transplantation. Mechanism of delaying protective effect after HPC correlate to production of HSP70.

Key words: heat shock precondition, pancreatic transplantation, ischemical reperfusion injury, heat shock protein 70