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低频经颅磁刺激对颞叶癫痫大鼠海马超微结构的影响

宋毅军1,田小冰1,田心2   

  1. 1. 天津医科大学总医院
    2. 天津医科大学
  • 收稿日期:2010-05-25 修回日期:2010-06-17 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 宋毅军

Effects of low frequency repetitive transcranial magnetic stimulation on the hippocampus ultrastructure of the temporal lobe epilepsy rats

yijun songTian xiao bing2,   

  • Received:2010-05-25 Revised:2010-06-17 Published:2010-11-15 Online:2010-11-15
  • Contact: yijun song

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摘要： 目的 低频重复经颅磁刺激（repetitive Transcranial Magnetic Stimulation, rTMS）能否成为治疗癫痫的新手段，其抗癫痫的作用机制到底是什么，是当前神经病学领域亟待解决的问题之一。用电镜从神经元、神经纤维、突触、胶质细胞和毛细血管五个方面观察颞叶癫痫对海马超微结构的病理损伤，研究低频经颅磁刺激（rTMS）对颞叶癫痫海马超微结构的影响。方法 制备氯化锂-匹罗卡品诱导的颞叶癫痫大鼠模型，应用电镜观察颞叶癫痫对海马超微结构的病理损伤和TMS对颞叶癫痫海马区病理损伤的作用。 结果 颞叶癫痫对海马区超微结构具有明显病理损伤，包括神经元出现凋亡和坏死，核浓缩，染色质聚集，线粒体嵴断裂，神经细胞和胶质细胞突起明显水肿，神经纤维局部髓鞘降解，轴浆内微管、微丝排列紊乱，血管内皮细胞肿胀，胶质细胞损伤。rTMS后海马超微结构明显改善，包括脑组织水肿减轻，凋亡和损伤细胞减少，突触和神经毡病变改善和血管水肿减轻。 结论 TMS可改善颞叶癫痫对海马超微结构的病理损伤，具有神经保护作用。

关键词: 复经颅磁刺激, 颞叶癫痫, 电镜, 海马, 超微结构

Abstract: Abstract: Objective：To study the effects of low frequency repetitive transcranial magnetic stimulation (rTMS) on the hippocampus ultrastructure of temporal lobe epilepsy（TLE）, by observing the pathological lesions of neurons, nerve fibers, synapses, glial cells and capillaries of TLE rats hippocampus with electron microscopy. Methods：Lithium-pilocarpine induced TLE rat models were randomly divided into two groups, TLE group and rTMS group. The ultrastructural changes in hippocampus of both groups were viewed with electron microscope. Results：TLE has significant pathological damage in the hippocampus ultrastructure, including neuronal apoptosis and necrosis, nuclear condensation, chromatin aggregation, mitochondrial cristae fracture, processes of nerve cells and glial cells edema, local medullary sheath of nerve fibers degradation, microtubule and microfilaments of axoplasm disarrangement, vascular endothelial cells swelling, glial cells demage. rTMS treatment can significantly improve the pathological damage, including relieving of tissue edema, reducing of apoptosis and damage cells, decreasing of synaptic and neuropil lesions, and alleviating of angioedema. Conclusion：TMS can relieve the pathological damage of TLE in the hippocampus ultrastructure and have neural protective effects in TLE.

Key words: repetitive Transcranial Magnetic Stimulation, Temporal lobe epilepsy, Hippocampus, Electron microscopy, Ultrastructure