天津医药

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抗氧化剂对ALI大鼠细胞因子的影响效应探讨

刘薇1,常诚2,康杰3,林书祥1,张慧1,赵津生1,郑捷1,马继军1,黄敬孚1   

  1. 1. 天津市儿童医院
    2. 天津市儿童医院心脏外科
    3. 天津市儿童医院内科监护病房
  • 收稿日期:2010-03-31 修回日期:2010-05-27 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 刘薇

Anti-oxidants on ALI in rats Effects of cytokines Discussion

  • Received:2010-03-31 Revised:2010-05-27 Published:2010-11-15 Online:2010-11-15

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摘要： 目的 探讨ALI时细胞因子的变化及抗氧化剂对ALI细胞因子的影响效应。方法 健康、成熟Wister大鼠60只随机分为2组，模型（对照）组和治疗（实验）组每组各30只，均以大肠杆菌腹腔注射，以氧合指数、肺湿/干重比、肺组织病理改变为标准，建立ALI动物模型。治疗（实验）组在注射大肠杆菌30min后，经大鼠尾静脉注射抗氧化剂——还原型谷胱甘肽。分别于注射大肠杆菌后3h，6h，12h测定血中IL-8、IL-10的变化。结果 模型（对照）组注射大肠杆菌后3h出现ALI症状，6h更为明显，12h口吐粉红色分泌物，解剖后肉眼所见双肺有明显水肿，出血点。氧合指数及W/D均达到ALI诊断标准。治疗（实验）组3h无明显变化，6h部分大鼠呼吸稍促，12h肺组织仅轻度水肿。治疗（实验）组肺湿/干比在3h、6h、12h检测值均低于模型（对照）组，6h、12h时差异存在统计学意义（p﹤0.05）。治疗（实验）组大鼠在使用还原型谷胱甘肽后，3h、6h、12h测得IL-8检测值（单位PG/ml）低于模型（对照）组，各时段差异均存在统计学意义（p﹤0.05）。治疗（实验）组大鼠在使用还原型谷胱甘肽后，3h、6h、12h测得IL-10检测值（单位PG/ml）高于模型（对照）组，6h、12h时差异均存在统计学意义（p﹤0.05）。结论 ALI时存在炎性介质的失控与失衡，应用抗氧化剂——还原型谷胱甘肽后可有效调控炎性介质，有助于减少超氧化物和溶酶体的释放、减轻炎性反应，从而降低ALI的发生几率。

关键词: 急性肺损伤, 白介素-8, 白介素-10, 抗氧化剂

Abstract: Objective To investigate the changes of cytokines during ALI and anti-oxidant effects of cytokines on the effects of ALI. Methods Healthy, mature, Wister rats were randomly divided into two groups 60, the model (control) group and treatment (experimental) group 30 rats in each group are based on intraperitoneal injection of E. coli in order to oxygenation index, lung wet / dry weight ratio, pathological changes in lung tissue as a standard, the establishment of animal models of ALI. Treatment (experimental) group at 30min after the injection of E. coli by rat tail vein injection of anti-oxidants - reduced glutathione. E. coli, respectively, after injection of 3h, 6h, 12h Determination of blood IL-8, IL-10 changes. Results Model (control) group 3h after injection of E. coli ALI symptoms occur, 6h more obvious, 12h vomit pink secretions, anatomical lung after the naked eye can see significant edema and bleeding. Oxygenation index and W / D reached the diagnostic criteria of ALI. Treatment (experimental) group 3h no significant change, 6h part of the rats were breathing a little promotion, 12h only a mild edema in lung tissue. Treatment (experimental) group the lung wet / dry than in the 3h, 6h, 12h test values are lower than the model (control) group, 6h, 12h, when differences were statistically significant (p <0.05). Treatment (experimental) group rats after the use of glutathione, 3h, 6h, 12h measured IL-8 detection value (units of PG / ml) lower than the model (control) group, there were statistical differences between each period significance (p <0.05). Treatment (experimental) group rats after the use of glutathione, 3h, 6h, 12h measured IL-10 measured values (in PG / ml) than the model (control) group, 6h, 12h, when differences exist in statistical significance (p <0.05). Conclusions Of inflammatory mediators in acute lung injury there is loss of control and balance, the application of anti-oxidants - reduced glutathione after the effective regulation of inflammatory mediators, contribute to reducing the release of super-oxide and lysosomes, reducing the inflammatory response , thereby reducing the risk of the occurrence of ALI.

Key words: Acute lung injury, Interleukin -8, Interleukin -10, anti-oxidants