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不同浓度臭氧水对口腔综合治疗台水系统中乙肝病毒作用研究?

杨鑫1,殷恺1,冯海2,王玉亮1   

  1. 1. 天津市第一中心医院
    2. 第一中心医院口腔科
  • 收稿日期:2011-11-08 修回日期:2012-02-17 出版日期:2012-06-15 发布日期:2012-06-15
  • 通讯作者: 杨鑫

Study on Different Concentrations of Ozone Solution Acting on the HVB Virus in Dental Unit Waterlines

  • Received:2011-11-08 Revised:2012-02-17 Published:2012-06-15 Online:2012-06-15
  • Contact: Xin YANG

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摘要： 摘要 目的：研究不同浓度臭氧溶液对口腔综合治疗台水道系统（DUWLs）中乙肝病毒（HBV）的灭活作用，探讨限时灭活HBV的最低臭氧溶液浓度。方法：常规培养2215细胞，在室温5℃、震荡混合、密闭等条件下配制不同浓度的臭氧溶液，将臭氧溶液加入2215细胞培养瓶中，作用不同时间后，取其上清液，提取HVB-DNA，聚合酶链式反应（PCR）扩增，荧光定量（FQ-PCR）检测HVB-DNA。结果：上述条件下配置的臭氧溶液浓度稳定，臭氧溶液能抑制2215细胞表达HBV-DNA，臭氧浓度为0.6 mg/L，10 min及0.5 mg/L，15 min能将设定浓度的HVB-DNA灭活。结论：0.5~0.6 mg /L臭氧水溶液可用于杀灭口腔综合治疗台水系统中的HBV。

关键词: 口腔综合治疗台水道系统, 臭氧, 乙肝病毒, 消毒, 生物膜

Abstract: Abstract Objective: To study different concentrations of ozone solution which acc on the Hepatitis b virus (HBV) in dental unit waterlines (DUWLs) and find out the minimum ozone water concentration which can inactivate HBV in DUWLs. Methods: Culture the human 2215 cell. Under the room temperature 5℃、concussion mixed、airtight and so on conditions, prepare different concentrations of aqueous solution of ozone.Put the ozone solution into the 2215 culture flask. Different concentrations of aqueous solution of ozone act with the supernatant liquid of the human 2215 cell in different times.Extract the HBV-DNA from the 2215 cell supernatant. Fluorescence quantitative PCR amplification. Detect the contents of HVB-DNA. Results: under those conditions, the aqueous solution of ozone is stable. The concentration of ozone at 0.6mg/L in 10 minutes and the concentration of ozone at 0.5mg/L in 15minet can inactivate Hepatitis b virus at limited level. Conclusion: The concentration of ozone at 0.5~ 0.6mg/L can inactivate Hepatitis b virus in DUWLs.

Key words: dental unit waterlines, ozone, hepatitis B virus, sterilize, biofilm