关键词: 灭菌；消毒；牙科合金；钛；葡萄球菌, 金黄色；TiO2涂层；抗菌

Abstract: Abstract： Objective To study the influence of different kinds of disinfection methods on antibacterial activity of TiO 2 nanotubes. Methods TiO2 nanotubes were fabricated with 0.5%wt HF on pure titanium to acid-etching combined with anodization as experimental group. The pure Ti was control group. They were disinfected by three kinds of disinfection meth⁃ ods, which were alcohol immersion, autoclaving and UV irradiation. The bacterial adhesion ability was evaluated by observ⁃ ing the morphology of samples, measuring the roughness and contracting angle. The film contact method was used to evaluate the antibacterial activity of samples with Staphylococcus aureus (Sa) after different infection treatment. The bacterial morphol⁃ ogy was observed by field emission scanning electron microscope (FE-SEM). Results The treatment group has higher sur⁃ face roughness. The bacterial adhesion ability of experimental group was stronger compared with that of control group. The contracting angle of samples exposed to UV irradiation was smaller than that of alcohol immersion and autoclaving treatment group. The viable count and FE-SEM showed that there were more bacteria and more complete morphology on the sample surface after alcohol immersion. The viable count was less and bacterial morphology suffered some damage on the sample sur⁃ face after high pressure steam sterilization. The least viable count and the most complete destruction of bacterial morphology were found after ultraviolet (UV) germicidal irradiation. Conclusion UV irradiation shows the best antibacterial activity, the second is autoclaving and the worst is alcohol immersion.

Key words: sterilization, disinfection, dental alloys, titanium, staphylococcus aureus, TiO 2 nanotubes, antibacterial