天津医药

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代谢综合征幼鼠血管VEGF、vWF的表达及意义

李亚璞1,刘戈力2,魏莹1,陈维彬1,高芳芳1   

  1. 1. 天津医科大学总医院
    2. 天津医科大学总医院儿科
  • 收稿日期:2011-05-25 修回日期:2011-07-26 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 刘戈力

Expression and Significance of VEGF，vWF in Vascular of Metabolic syndrome young rats

  • Received:2011-05-25 Revised:2011-07-26 Published:2012-02-15 Online:2012-02-15

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摘要： 摘要：目的：探讨血管内皮生长因子（VEGF），血管性假血友病因子（vWF）在代谢综合征（MS）幼鼠血管壁和血清中的表达及意义。方法：3周龄SD大鼠随机分为普通饮食组（NC组）、高脂组（FC组）、高脂＋高盐组（FSC组）。实验4周末测体质量、腹围、血压，内脏脂肪质量、血脂、行OGTT实验，计算胰岛素抵抗指数。取腹主动脉行HE染色，免疫组化法检测血管壁VEGF，vWF表达，Elisa方法检测血清VEGF，vWF表达。结果：FSC组体重，腹围，血压，内脏脂肪，血糖，胰岛素水平均较NC组增加，血脂紊乱加重，并出现胰岛素抵抗。HE染色显示FSC组血管壁局部平滑肌细胞排列紊乱，血管壁及血清中VEGF，vWF均升高。结论：本实验以高脂高盐饮食成功诱导建立幼鼠MS模型，血管壁及血清VEGF, vWF表达损伤的增加，表明MS幼鼠血管壁已出现功能紊乱或损伤。VEGF, vWF参与了其损伤的病生理改变

关键词: 代谢综合征, 胰岛素抵抗, 血管内皮生长因子, 血管性假血友病因子, 血管疾病模型, 动物, 大鼠, Sprague-Dawley

Abstract: Abstract：Objective：To investigate the effect of the expression of VEGF 、vWF on vascular wall of Metabolic syndrome young rats . Methods：Four-week-old SD rats (50 g body weight) were randomly divided into 3 groups, routine diet group (NC), high fat diet group (FC) and high fat + high salt diet group (FSC). The values of body weight, abdominal circumference, blood pressure, visceral fat weight, plasma lipids, were measured 4 weeks after experiments. The levels of fasting plasma glucose and insulin were measured, and the insulin resistance index was calculated. HE stain and immunohistochemistry were used to detect the pathologic change and the infiltration cells with positive expression of VEGF，vWF in the abdominal aorta, Use elisa method to detect serum VEGF，vWF. Results: The values of body weight, abdominal circumference, blood pressure, visceral fat, plasma glucose and insulin level were significantly increased in the FSC group than that of the NC group. There were increased dyslipidemia and insulin resistance in the FSC group compared with those of the NC group. The abdominal aorta and serum express VEGF、vWF of FSC group were significantly increased than that of the NC，FC group. (p＜0.01). Conclusion: The rat model of metabolic syndrome was successfully established by high fat+salt diet in juvenile SD rats. The high expression of VEGF、vWF may be related to the occurrence and development of vascular pathology and physiological changes of metabolic syndrome in children.

Key words: metabolic syndrome, insulin resistance , VEGF , vWF , Vasculardisease models, animal , rats, Sprague-Dawley