棕矢车菊素调节SDF-1α/CXCR4信号通路对妊娠糖尿病大鼠炎症反应的影响

doi:10.11958/20231228

天津医药 ›› 2024, Vol. 52 ›› Issue (6): 594-598.doi: 10.11958/20231228

棕矢车菊素调节SDF-1α/CXCR4信号通路对妊娠糖尿病大鼠炎症反应的影响

蒋韬(), 程红艳, 吴琼()

随州市中心医院妇产科（邮编441300）

收稿日期:2023-08-11 修回日期:2023-10-24 出版日期:2024-06-15 发布日期:2024-06-06
通讯作者: ^△E-mail：qmrdcztchao3@163.com
作者简介:蒋韬（1985），女，主治医师，主要从事高危妊娠方面研究。E-mail：muuuuuv@163.com
基金资助:
随州市卫生和计划生育委员会资助项目(2018SZ32008)

Effect of Jaceosidin on inflammatory response in gestational diabetes mellitus rats by regulating SDF-1α/CXCR4 signaling pathway

JIANG Tao(), CHENG Hongyan, WU Qiong()

Department of Obstetrics and Gynecology, Suizhou Central Hospital, Suizhou 441300, China

Received:2023-08-11 Revised:2023-10-24 Published:2024-06-15 Online:2024-06-06
Contact: ^△E-mail： qmrdcztchao3@163.com

蒋韬, 程红艳, 吴琼. 棕矢车菊素调节SDF-1α/CXCR4信号通路对妊娠糖尿病大鼠炎症反应的影响[J]. 天津医药, 2024, 52(6): 594-598.

JIANG Tao, CHENG Hongyan, WU Qiong. Effect of Jaceosidin on inflammatory response in gestational diabetes mellitus rats by regulating SDF-1α/CXCR4 signaling pathway[J]. Tianjin Medical Journal, 2024, 52(6): 594-598.

摘要/Abstract

摘要：

目的探讨棕矢车菊素对妊娠糖尿病（GDM）大鼠炎症反应的影响及可能机制。方法将妊娠大鼠腹腔注射链脲佐菌素建立GDM模型，将建模成功大鼠分为GDM组，棕矢车菊素-L、M、H组，棕矢车菊素+CTCE-0214[基质细胞衍生因子1α（SDF-1α）/趋化因子受体4（CXCR4）激活剂]组，每组10只；另取10只健康妊娠大鼠作为对照组。棕矢车菊素-L、M、H组分别灌胃2、3、4 mg/kg棕矢车菊素，棕矢车菊素+CTCE-0214组腹腔注射10 mg/kg CTCE-0214并灌胃4 mg/kg棕矢车菊素，对照组和GDM组给予等量生理盐水。比较各组大鼠空腹血糖、空腹胰岛素以及胰岛素抵抗指数差异；酶联免疫吸附测定法检测大鼠胎盘组织中白细胞介素（IL）-1β、肿瘤坏死因子（TNF）-α水平；HE染色观察胎盘组织病理变化；TUNEL检测胎盘组织细胞凋亡；Western blot检测大鼠胎盘组织中SDF-1α/CXCR4通路相关蛋白水平。结果不同剂量棕矢车菊素干预GDM大鼠引起大鼠空腹血糖、空腹胰岛素、胰岛素抵抗指数水平降低，促炎细胞因子水平降低，改善GDM大鼠胎盘损伤和细胞凋亡，并抑制SDF-1α/CXCR4信号通路激活，呈剂量依赖性（P<0.05），SDF-1α/CXCR4激活剂和棕矢车菊素联合干预后抑制棕矢车菊素上述作用。结论棕矢车菊素可通过抑制SDF-1α/CXCR4信号通路激活来减轻GDM大鼠炎症反应和胰岛素抵抗。

关键词: 糖尿病, 妊娠, 疾病模型, 动物, 趋化因子CXCL12, 炎症, 棕矢车菊素, 趋化因子配体4

Abstract:

Objective To investigate the effect of Jaceosidin on inflammatory response in gestational diabetes mellitus (GDM) rats and its mechanism. Methods The GDM rat model was established by intraperitoneal injection of streptozotocin into pregnant rats. The successfully established rats were separated into the GDM group, the Jaceosidin L, M, H groups and the Jaceosidin+CTCE-0214 [stromal cell-derived factor-1α (SDF-1α)/C-X-C chemokine receptor 4 (CXCR4) activator] group, with 10 rats in each group. Meantime, 10 healthy pregnant rats were taken as the control group. The Jaceosidin L, M and H groups were gavaged with 2, 3 and 4 mg/kg Jaceosidin, respectively. The Jaceosidin+CTCE-0214 group was intraperitoneally injected with 10 mg/kg CTCE-0214 and gavaged with 4 mg/kg Jaceosidin. The control group and the GDM group were given equal amounts of physiological saline. The fasting blood glucose, fasting insulin and insulin resistance index were compared between each group of rats. Enzyme-linked immunosorbent assay was applied to detect levels of IL-1β and TNF-α in placental tissue. HE staining was applied to observe pathological changes of placental tissue. Apoptosis of placental tissue was detected by TUNEL. Western blot assay was applied to detect levels of SDF-1α/CXCR4 pathway related proteins in placental tissue. Results The intervention of different doses of Jaceosidin in GDM rats reduced levels of fasting blood glucose, fasting insulin, insulin resistance index and proinflammatory cytokines, improved the placental injury and apoptosis of GDM rats, and inhibited the activation of SDF-1α/CXCR4 signaling pathway in a dose-dependent manner (P<0.05). SDF-1α/CXCR4 activator combined Jaceosidin could inhibit the above effects of Jaceosidin. Conclusion Jaceosidin can reduce inflammation and insulin resistance in GDM rats by suppressing the activation of SDF-1 α/CXCR4 signaling pathway.

Key words: diabetes, gestational, disease models, animal, chemokine CXCL12, inflammation, Jaceosidin, chemokine ligand 4

中图分类号:

R587.1，R714.256

图/表 6

参考文献 22

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组别	空腹血糖/ （mmol/L）	空腹胰岛素/ （mIU/L）	胰岛素抵抗指数
对照组	7.41±0.89	4.14±0.53	1.36±0.15
GDM组	16.45±1.02^a	13.56±1.54^a	9.91±1.02^a
棕矢车菊素-L组	14.33±1.55^b	10.85±1.11^b	6.91±0.74^b
棕矢车菊素-M组	11.18±1.29^bc	8.65±0.94^bc	4.29±0.45^bc
棕矢车菊素-H组	9.16±0.94^bcd	6.34±0.66^bcd	2.58±0.28^bcd
棕矢车菊素+ CTCE-0214组	15.11±1.66^e	11.32±1.26^e	7.12±0.81^e
F	80.846^＊＊	106.534^＊＊	240.593^＊＊

组别	空腹血糖/ （mmol/L）	空腹胰岛素/ （mIU/L）	胰岛素抵抗指数
对照组	7.41±0.89	4.14±0.53	1.36±0.15
GDM组	16.45±1.02^a	13.56±1.54^a	9.91±1.02^a
棕矢车菊素-L组	14.33±1.55^b	10.85±1.11^b	6.91±0.74^b
棕矢车菊素-M组	11.18±1.29^bc	8.65±0.94^bc	4.29±0.45^bc
棕矢车菊素-H组	9.16±0.94^bcd	6.34±0.66^bcd	2.58±0.28^bcd
棕矢车菊素+ CTCE-0214组	15.11±1.66^e	11.32±1.26^e	7.12±0.81^e
F	80.846^＊＊	106.534^＊＊	240.593^＊＊

组别	IL-1β/（ng/L）	TNF-α/（ng/L）	凋亡率/%
对照组	23.41±2.53	34.15±3.26	1.26±0.13
GDM组	66.45±6.94^a	96.55±10.14^a	13.52±1.42^a
棕矢车菊素-L组	54.33±5.61^b	80.84±8.69^b	10.62±1.10^b
棕矢车菊素-M组	42.18±4.28^bc	65.61±6.28^bc	7.61±0.85^bc
棕矢车菊素-H组	34.12±3.18^bcd	46.34±4.58^bcd	4.62±0.62^bcd
棕矢车菊素+ CTCE-0214组	56.41±5.76^e	84.25±8.64^e	11.32±1.26^e
F	102.559^＊＊	106.091^＊＊	212.626^＊＊

组别	IL-1β/（ng/L）	TNF-α/（ng/L）	凋亡率/%
对照组	23.41±2.53	34.15±3.26	1.26±0.13
GDM组	66.45±6.94^a	96.55±10.14^a	13.52±1.42^a
棕矢车菊素-L组	54.33±5.61^b	80.84±8.69^b	10.62±1.10^b
棕矢车菊素-M组	42.18±4.28^bc	65.61±6.28^bc	7.61±0.85^bc
棕矢车菊素-H组	34.12±3.18^bcd	46.34±4.58^bcd	4.62±0.62^bcd
棕矢车菊素+ CTCE-0214组	56.41±5.76^e	84.25±8.64^e	11.32±1.26^e
F	102.559^＊＊	106.091^＊＊	212.626^＊＊

组别	SDF-1α	CXCR4
对照组	0.34±0.04	0.50±0.05
GDM组	1.48±0.15^a	1.65±0.18^a
棕矢车菊素-L组	1.12±0.13^b	1.26±0.13^b
棕矢车菊素-M组	0.73±0.09^bc	0.99±0.09^bc
棕矢车菊素-H组	0.47±0.06^bcd	0.67±0.07^bcd
棕矢车菊素+CTCE-0214组	1.15±0.12^e	1.30±0.14^e
F	173.952^＊＊	130.277^＊＊

棕矢车菊素调节SDF-1α/CXCR4信号通路对妊娠糖尿病大鼠炎症反应的影响

Effect of Jaceosidin on inflammatory response in gestational diabetes mellitus rats by regulating SDF-1α/CXCR4 signaling pathway

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