天津医药

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缺氧培养骨髓内皮祖细胞移植对缺血性心脏病的治疗作用

简锴陶1,王联群1,王强1,郑宝荣2,刘建实3   

  1. 1. 天津市胸科医院
    2. 天津医科大学
    3. 天津市胸科医院心外科
  • 收稿日期:2012-07-11 修回日期:2012-10-02 出版日期:2013-02-15 发布日期:2013-02-15
  • 通讯作者: 简锴陶

The therapeutic effect on ischemia heart disease by hypoxic preconditioning of endothelial progenitor cells transplantation

JIAN Kai tao1,WANG Lian qun1,WANG Qiang 1,Bao-rong Zheng2,LIU Jianshi 1   

  1. 1. Tianjin Chest Hospital, Tianjin 300051, China
    2.
  • Received:2012-07-11 Revised:2012-10-02 Published:2013-02-15 Online:2013-02-15
  • Contact: JIAN Kai tao

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摘要：

【摘要】目的 探讨缺氧培养的骨髓内皮祖细胞心肌内移植治疗缺血性心脏病的效果。方法 从8周龄同种系成年雄性Wistar大鼠长骨中获取骨髓内皮祖细胞，常规培养4 d后，1%O2+5%CO2+94%N2培养3 d。将26只8周龄同种系成年雄性Wistar大鼠随机分为对照组（n=8）、常规组（n=9）和缺氧组（n=9),建立急性心肌梗死动物模型。在心肌梗死边缘5个不同区域心肌内分别注射PBS溶液200 μL，内皮祖细胞2×106个，缺氧培养内皮祖细胞2×106个，4周后应用压力－容量曲线分析评估实验动物血流动力学和心脏功能变化。结果 缺氧培养骨髓内皮祖细胞心肌内移植4周后，缺氧组与对照组相比较，舒张末容积、收缩末容积、心搏量、射血分数以及每分心输出量改善明显，差异均有统计学意义；缺氧组与常规组相比，收缩末容积减小，心搏量、射血分数及每分心输出量提高，差异均有统计学意义；常规组与对照组比较，心搏量、射血分数和每分心输出量无明显改善，差异无统计学意义。结论 缺氧培养大鼠骨髓内皮祖细胞较常规培养细胞对缺血性心脏病的治疗效果更加明显，限制了心室重构，改善了梗死后整体的心脏功能。

关键词: 缺氧培养, 骨髓内皮祖细胞, 缺血性心脏病, 细胞治疗, 压力－容量曲线

Abstract:

[Abstract] Objective  To investigate the therapeutic effectiveness of transplantating hypoxic preconditioning endothelial progenitor cells (HEPCs) on acute myocardial infarction. Methods  Bone marrow endothelial progenitor cells (EPCs) were isolated from 8-week syngeneic adult male Wistar rats. After normoxic culture for 4 days, EPCs were cultured in 1% O2+ 5% CO2+94% N2 for 3 days. Then, 26 8-week syngeneic adult male Wistar rats were randomized into 3 groups: control group (n=8)，EPCs group (n=9) and HEPCs group (n=9). The acute myocardial infarction animal model was set up. Rats received 5-points peri-infarct intramyocardial injections of PBS 200 μL 2×106 EPCs and 2×106 HEPCs. After 4 weeks, the pressure-volume (P-V) curve analysis was used to evaluate the haemodynamics parameters and cardiac function. Results  After 4 weeks of transplantation, P-V loop analysis showed that there were significant differences in end diastolic volume, end systolic volume, stroke volume,ejection fraction( EF) and cardiac output between HEPCs and control groups. There were significant differences in end systolic volume, stroke volume and cardiac output between HEPCs and EPCs groups. There were no significant differences in stoke volume and cardiac output between EPCs and control groups. Conclusion Transplantation of HEPCs limited left ventricular remodeling and improved cardiac function entirely after acute myocardial infarction.

Key words: Hypoxic preconditioning, Bone marrow endothelial progenitor cells, Ischemia heart disease, Cell therapy, Pressure-volume loops