天津医药

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miR-224和 miR-378e在大肠癌组织中的表达及其临床意义

高利飞1,田延锋2,赵增仁2,张丽静2,贺新奇2,裴永彬2   

  1. 1. 河北医科大学第一医院普外科
    2. 河北医科大学第一医院
  • 收稿日期:2012-11-01 修回日期:2013-03-13 出版日期:2013-08-15 发布日期:2013-08-15
  • 通讯作者: 赵增仁

GAO Lifei ,TIAN Yanfeng ,ZHAO Zengren ,ZHANG Lijing ,HeXin-Qi ,PEI Yongbin   

  1. Department of General Surgery, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China
  • Received:2012-11-01 Revised:2013-03-13 Published:2013-08-15 Online:2013-08-15
  • Contact: ZHAO Zengren

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摘要： 【摘要】 目的  探讨miR-224 和 miR-378e 在原位大肠癌癌组织和癌旁正常黏膜组织中的表达差异， 并分析其临床意义。 方法 miRNA 表达谱芯片技术检测大肠癌癌组织和癌旁组织标本中表达差异的miRNA， 运用荧光实时定量聚合酶链反应（real-time PCR）验证其结果，并分析其与临床病理资料之间的关系。 结果 miRNA 表达芯片分析发现， 与正常黏膜组织比较， miR-224在大肠癌组织中表达显著上调， miR-378e在大肠癌组织中表达显著下调， 并被 real-time PCR 所证实。 miR-224 在不同组织学类型癌组织中表达差异有统计学意义， miR-378e 在不同浸润深度癌组织中表达差异有统计学意义， miR-224 表达与组织学类型有关， miR-378e 表达与大肠癌浸润深度有关。 结论miR-224具有潜在的癌基因作用， miR-378e具有潜在的抑癌基因作用， miR-224和 miR-378e可作为

关键词: 结直肠肿瘤, 癌, 微RNAs, 寡核苷酸序列分析, 聚合酶链反应, miR-224, miR-378e

Abstract: Objective    To investigate the expression and clinical significance of microRNA-224 and microRNA -378e in non-metastatic colonrectal cancer tissues and non-cancerous tissues surrounding colonrectal cancer in situ .Methods   The expression level of miR-224 and miR-378e in tumor and adjacent normal tissue from clinical colonrectal cancer patients without metastasis was detected by Chip - array . And the determination results were verified by real - time PCR . The correlation of miR-224 and miR-378e expession and clinicopathological features of colonrectal cancer was analyzed . Results   The expression level of miR-224 was significantly up-regulated in tumor tissue (P<0 .001)while miR-378e was down-regulated in tumor tissue , and validated by real-time PCR . The expression of miR-224 was strongly associated with Histological type(P<0 .05)，while miR-378e with Infiltration depth(P<0 .05) . Conclusion   miR-224 is a potent tumor promoter , while miR-378e is a potent tumor suppressor .

Key words: colorectal neoplasms, carcinoma, microRNAs, oligonucleotide array sequence analysis, polymerasechain reaction, miR-224, miR-378e