天津医药

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干扰素体外干预对肾癌组织STAT1和P-STAT1表达的影响

李伟,梁建波   

  1. 广西壮族自治区人民医院泌尿外科
  • 收稿日期:2013-03-09 修回日期:2013-06-15 出版日期:2013-10-15 发布日期:2013-10-15
  • 通讯作者: 李伟

  • Received:2013-03-09 Revised:2013-06-15 Published:2013-10-15 Online:2013-10-15
  • Contact: Wei LI

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摘要：

【摘要】 目的  研究干扰素体外干预对肾癌组织STAT1和P-STAT1蛋白表达的影响。  方法  收集新鲜人肾细胞癌组织标本8例，使用干扰素（INF）α-2b对肿瘤组织进行体外干预，Western-blot检测STAT1及P-STAT1蛋白的相对表达量，并比较干预前后表达量的变化。  结果  加入IFNα-2b后，标本中的P-STAT1表达量增高（0.18±0.10vs0.44±0.26，P＜0.05），而STAT1变化不大（0.40±0.28vs0.37±0.28，P>0.05）。结论    干扰素体外干预可提高P-STAT1在部分肾癌组织中的表达，其表达变化可能成为评估干扰素疗效的指标。

关键词: 癌, 肾细胞, STAT1转录因子, 干扰素α-2b

Abstract: Objective: To research The expression of STAT1 and P-STAT1 in renal cancer tissue after interferon intervention in vitro. Methods:8 cases of fresh renal cancer tissue were collected and intervened with interferonα-2b, the expression of STAT1 and P-STAT1 were detected by western-blot. The relative amount of expression was compared. Results: The expression of P-STAT1 increased in 6 cases after intervention with interferon. The expression of STAT1 slight increased in 2 cases. Conclusion: The expression of P-STAT1 increased in some renal cancer tissues after intervention with interferon. Vary of expression maybe predicts the effect of immunotherapy.