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低形态学评分卵裂期胚胎的X、Y及18号染色体分析

罗海宁1,杨清秀2,鲁振宇3,张云山3,杜湧瑞1,王毅娜1,姚汝强1   

  1. 1. 天津市中心妇产科医院
    2. 中心妇产科医院生殖医学中心
    3. 天津市中心妇产科医院生殖医学中心
  • 收稿日期:2010-06-29 修回日期:2010-11-27 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 杨清秀

Analysis of Chromosome X, Y and 18 in Cleavage Stage Embryos with Low Morphological Scores by the Fluorescent in situ Hybridization

  • Received:2010-06-29 Revised:2010-11-27 Published:2011-04-15 Online:2011-04-15

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摘要： 摘要 目的：应用荧光原位杂交技术分析低形态学评分的D3卵裂期胚胎X、Y、18号染色体的非整倍体情况。方法：收集体外受精-胚胎移植术后剩余的废弃胚胎，整个胚胎固定后采用荧光原位杂交技术检测细胞核内的X、Y和18号染色体。结果：观测到荧光信号的细胞核有233个，其中正常细胞核115个（49.4%），异常细胞核118个（50.6%）。18号染色体非整倍体率40.8%，X/Y染色体非整倍体率33.5%，两者之间的差别无统计学意义（P>0.05）。18号染色体三体发生率为21.9%（n=51）显著高于X/Y染色体12.0%（n=28）（P<0.01）。结论：D3形态评分低的胚胎有相当大的比例存在染色体异常，通用的胚胎形态学评分系统能够在一定程度上反映胚胎的染色体情况。

关键词: 非整倍体, 荧光原位杂交, 卵裂期胚胎, 形态学评分

Abstract: ABSTRACT Objective: To evaluate the aneuploidy status of chromosome X, Y and 18 in day 3 cleavage stage embryos with low morphological scores by the fluorescent in situ hybridization. Methods: The discarded embryos from IVF-ET cycles were collected for our research. Nuclei of all the blastomeres were fixed in which the chromosome X, Y and 18 were analyzed by FISH. Results: Fluorescent signals were successfully detected in 233 nuclei, in which 115 (49.4%) nuclei were normal euploidy of chromosome X, Y and 18, while 118 (50.6%) were abnormal. The aneuploidy rate of chromosome 18 and X/Y were 40.8% and 33.5% respectively, but the differences was not statistical significant. The trisome rate of chromosome 18 was statistical higher than that of chromosome X/Y (21.9%vs.12.0%, P<0.01). Conclusion: A majority of the day 3 cleavage stage embryos with low morphological scores were proved to be chromosomal abnormal. The general embryo morphology scoring criteria could predict the embryo euploidy status to some extent.

Key words: Aneuploidy, Fluorescence in situ hybridization, Cleavage stage embryo Embryo, morphology scoring