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CD44抗体对卵巢癌实体瘤源球形体形成细胞增殖的抑制作用

贺晓英1,王翠环2,邓为民3   

  1. 1. 天津医科大学免疫学教研室1005室
    2. 天津医科大学基础医学院免疫教研室
    3. 天津医科大学免疫教研室
  • 收稿日期:2010-10-29 修回日期:2010-12-26 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 贺晓英

The effects and mechanisms of monoclonal antibody against CD44 (A3D8) on proliferation in sphere-forming cells from solid tumor of ovarian cancer

  • Received:2010-10-29 Revised:2010-12-26 Published:2011-04-15 Online:2011-04-15
  • Contact: Xiao-Ying HE

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摘要： 摘要 目的: 观察抗CD44单克隆抗体A3D8对卵巢癌实体瘤源球形体形成细胞增殖的作用,并初步探讨其作用机理。方法: 用MTS法检测A3D8及顺铂(DDP)对细胞增殖的影响；采用流式细胞仪法观察A3D8对卵巢癌实体瘤源球形体形成细胞细胞周期的影响；彗星电泳法检测DNA损伤；通过Western blot 法，进一步探讨A3D8作用于卵巢癌实体瘤源球形体形成细胞后CDK2、cyclinA蛋白水平的表达变化。结果: A3D8作用于卵巢癌实体瘤源球形体形成细胞后，细胞抑制率明显升高，而且随着A3D8浓度的增加和时间的延长，抑制作用明显增强（P<0.05），与DDP联用，抑制率比单独用DDP增高； A3D8可将细胞周期阻滞于S期（P<0.01）；A3D8作用于卵巢癌实体瘤源球形体形成细胞24h后引起DNA损伤；A3D8组CDK2、cyclinA蛋白表达水平明显降低（P <0.05）。结论: A3D8可能通过阻滞细胞周期发挥抑制卵巢癌实体瘤源球形体形成细胞增殖的作用。

关键词: 抗CD44单克隆抗体A3D8, 卵巢癌实体瘤源球形体形成细胞, 细胞增殖

Abstract: Abstract Objective: To explore the effects and mechanisms of monoclonal antibody against CD44 (A3D8) on proliferation in sphere-forming cells from solid tumor of ovarian cancer. Methods: MTS assay was used to evaluate cell proliferation; flow cytometry was used to analyze cell cycle distribution; DNA damage was measured by comet assay; expression of CDK2 and cyclinA proteins after treatment with A3D8 was evaluated by western blot assay. Results: A3D8 could inhibit the proliferation of sphere-forming cells from solid tumor of ovarian cancer; the inhibition was in dose- and time-dependant manner; compared with treatment with DDP alone, the inhibition rate caused by A3D8 combined with DDP was increased significantly ;The percentage of cells in S phase significantly increased after treatment with A3D8;DNA was damaged after treatment with A3D8 for 24h; western blot analysis demonstrated that the expression of CDK2、cyclinA proteins was suppressed significantly. Conclusion: A3D8 could inhibit the proliferation of sphere-forming cells from solid tumor of ovarian cancer, and the inhibiting effect was probably carried out by arrest of cell cycle.

Key words: monoclonal antibody against CD44 (A3D8), sphere-forming cells from solid tumor of ovarian cancer, cell proliferation