天津医药

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生长分化因子9与超排卵周期卵巢低反应的相关性研究

王会妍1,糜若然2,3,张云山4   

  1. 1. 天津医科大学总医院
    2. 天津医科大学总医院妇产科
    3.
    4. 天津市中心妇产科医院生殖医学中心
  • 收稿日期:2010-10-22 修回日期:2010-12-29 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 王会妍

The correlation between GDF-9 expression in granulosa cells and poor ovarian response to controlled ovarian hyper stimulation

  • Received:2010-10-22 Revised:2010-12-29 Published:2011-04-15 Online:2011-04-15

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摘要： 目的：研究超排卵周期卵巢低反应与颗粒细胞中生长分化因子-9（GDF-9）表达的相关性。方法：对70例行体外受精-胚胎移植（IVF-ET）患者，采用本中心常规超排卵方案，应用实时荧光定量PCR（RQ-PCR）方法检测卵巢不同反应组患者卵泡壁颗粒细胞和卵丘颗粒细胞GDF-9 mRNA表达，分析其与卵巢低反应的关系。结果：卵巢不同反应组间患者年龄、不孕年限、基础FSH、E2水平及Gn用量差异均无统计学意义（P＞0.05）。卵巢低反应组卵泡壁颗粒细胞和卵丘颗粒细胞GDF-9 mRNA表达分别为18.64±8.35；19.29±9.32，均低于中反应组及高反应组（P＜0.05），且GDF-9表达与获卵数呈正相关（r=0.508；0.632，P＜0.01）。结论: 卵巢低反应与颗粒细胞GDF-9表达有关，其表达水平降低则在超排卵周期中发生卵巢低反应的可能性增加。

关键词: 超排卵, 生长分化因子-9, 体外受精-胚胎移植, 卵巢低反应

Abstract: Abstract Objective: To determine whether the expression of GDF-9 in granulosa cells was related to poor ovarian response to gonadotropin stimulation. Methods: RQ-PCR was performed to detect the levels of GDF-9 mRNA in granulose cells obtained from 70 patients undergoing IVF treatment, the relation was analyzed between the level of GDF-9 and poor ovarian response. Results: There were not significant differences with patient age, the serum levels of basal follicle-stimulating hormone and basal estradiol, years of infertility, dosages of gonadotropin in different ovarian response groups（P＞0.05）. The expression of GDF-9 mRNA in the poor ovarian response group was lower than those of moderate and high responders, being 18.64±8.35, 19.29±9.32 on mural and cumulus granulose cells, respectively(P＜0.05). The GDF-9 expression was positively correlated with the number of follicles (r=0.508; 0.632, P＜0.01). Conclusion: The lower expression level of GDF-9 in granulosa cells may be associated with poor response to controlled ovarian hyper stimulation.

Key words: Superovulation, growth differentiation factor-9, In vitro fertilization-embryo transfer, poor ovarian response