天津医药

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黏附分子L1在胃肠道间质瘤中表达及其临床意义

姜忠敏1,成元华2,王淑妍2   

  1. 1. 天津市第五中心医院
    2. 贵阳医学院病理科
  • 收稿日期:2011-07-19 修回日期:2012-02-08 出版日期:2012-08-15 发布日期:2012-08-15
  • 通讯作者: 姜忠敏

Expression and clinical significance of adhesion molecule L1 in gastrointestinal stromal tumors

  • Received:2011-07-19 Revised:2012-02-08 Published:2012-08-15 Online:2012-08-15

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摘要： 摘要　目的：探讨黏附分子L1在胃肠道间质瘤(GIST)中的表达情况及其临床意义。方法：　采用RT-PCR和免疫组织化学法分别检测66例GIST、10例平滑肌肿瘤、6例神经鞘瘤及14例正常肠壁组织中L1蛋白及mRNA的表达，并对患者进行随访。分析L1表达与GIST临床病理特征间的关系及其在GIST、平滑肌瘤、神经鞘瘤中表达差异。结果：胃肠道间质瘤中L1蛋白及mRNA的表达率分别为57.6%(38/66)、 83.3% (30/36) , 且二者间差异有显著性( P <0.05); L1表达在GIST不同侵袭危险组间比较差异有显著性(P<0.05)，但与性别、年龄、部位、组织类型及患者预后均无明显相关性 (P>0.05)。L1在GIST中的表达明显高于其在平滑肌肿瘤及正常肠组织中的表达 ( P <0.05) ，与其在神经鞘瘤中的表达无明显差异。 结论：L1表达在GIST发生发展过程中起促进作用,提示L1可作为GIST 的诊断和鉴别诊断指标；能否评估预后尚需进一步研究。

关键词: 胃肠道间质瘤, L1, 免疫组织化学, 反转录-聚合酶链式反应

Abstract: Abstract　Objective: To investigate the expression of L1 in gastrointestinal stromal tumors (GIST)and its significance. Methods: L1 Protein and mRNA were detected in the tissues of 66 cases of GISTs , 10 cases of smooth muscle tumors, 6 cases of schwannoma and 14 cases of the normal bowel by immunohistochemistry and RT-PCR. And the follow up was carry out.The correlation of L1 expression with clinical pathologic features of GIST and the difference L1 expression in between GIST and smooth muscle tumors, schwannoma, the normal bowel tissue were analyzed. Results:　The expression rates of L1 Protein and mRNA in GISTs were 57.6%(38/66) and 83.3% (30/36) , and the difference of the both was significant ( P < 0.05). the expression of L1 had significant difference in different aggressive risk of GIST(P<0.05),but had no correlation with age，sex ,locus, tissue type and survival rate in GIST（P>0.05）. L1 expression in GIST was significant higher than it in smooth muscle tumors and the normal bowel tissue ( P < 0.05) and had no significant difference with it in schwannoma. Conclusion: The expression of L1 may play an important role in tumorigenesis and progression of GIST, which suggest that L1 is applied to diagnosis or differential diagnosis of GIST; Future studies might focus on the impact of the expression of L1on survival.

Key words: gastrointestinal stromal tumors, L1, immunohistochemistry, Reverse transcription polymerase chain reaction