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3-氨基苯硼酸纸片法检测大肠埃希菌高产AmpC酶的探讨

李静1,胡志东2,田 彬2,徐海茹2,李金2   

  1. 1. 天津医科大学总医院
    2. 天津医科大学总医院检验中心
  • 收稿日期:2011-04-28 修回日期:2011-06-29 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 李静

3-aminophenylboronic acid disk synergy test for identication of Class C β-lactamase-producing Escherichia coli

  • Received:2011-04-28 Revised:2011-06-29 Published:2011-12-15 Online:2011-12-15

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摘要： AmpC酶又称诱导酶，属于Bush分类中的Ⅰ类酶及分子生物学中的C类酶[1]。AmpC酶多数由染色体介导，部分呈诱导型表达。近年也陆续发现了质粒介导AmpC酶，其耐药基因可在同种或不同种属细菌间广泛传播，使细菌耐药日趋复杂，给临床治疗提出了严峻的挑战，迫切需要临床实验室能快速而准确地检测产AmpC酶细菌。目前临床实验室标准化委员会（CLSI）尚未确定检测的标准方法，本研究以三维试验和聚合酶链反应（PCR）为参照，与3-氨基苯硼酸（APB）双纸片协同法进行比较，以评价APB双纸片协同法用于临床实验室检测临床分离的大肠埃希菌的可靠性。

关键词: 大肠杆菌, β内酰胺酶类, 纸片扩散抗菌试验, 聚合酶链反应, 微生物敏感性试验

Abstract: Objective： To establish and evaluate a new method to detect high producing AmpCβ-lactamase Escherichia coli.Methods:AmpC β-lactamase from 44 strains of cefoxitin-resistant Escherichia coli were detected by 3-aminophenylboronic acid (APB) disk synergy test in comparison with three-dimensional test and polymerase chain reaction (PCR). The results were analysed by statistics analysis method. Result: In 44 strains of cefoxitin-resistant Escherichia coli, AmpC β-lactamase positive rates of three methods were 70.5%，56.8% and 75% respectively.Compared with three-dimensional test, the result showed that the positive coincidence rate、negtive coincidence rate and total coincidence rate were 88.0%，52.6% and 72.7% respectively，there were no differences between APB disk synergy test and three-dimensional test（P>0.05）. Compared with PCR，the result showed that the sensitivity, specificity and the coincidence rate were 90.1%，90.1% and 90.1% respectively, there were no differences between APB disk synergy test and PCR method（P>0.05）. Conclusions:APB disk synergy test is simple,correct,not demanding special equipments. Thus ,it is applicable for routine use in clinical microbiology laboratoryes.