天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (6): 687-690.doi: 10.11958/20150281

• 细胞与分子生物学 • 上一篇    下一篇

大蒜素抑制牙龈卟啉单胞菌诱导人肺上皮细胞凋亡的作用

孟田甜， 李新   

  1. 锦州医科大学附属口腔医院修复科
  • 收稿日期:2015-11-10 修回日期:2016-03-22 出版日期:2016-06-15 发布日期:2016-07-04
  • 基金资助:
    辽宁省自然科学基金项目 （2013022029）； 辽宁医学院校长基金 （QM2014017）

The inhititory effects of allicin on human lung epithelial cell apoptosis induced by porphyromonas gingivalis

MENG Tiantian, LI Xin   

  1. Department of Prosthetics, School of Stomatology, Jinzhou Medical University, Jinzhou 121000, China
  • Received:2015-11-10 Revised:2016-03-22 Published:2016-06-15 Online:2016-07-04

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摘要： 摘要： 目的 探讨不同浓度大蒜素对牙龈卟啉单胞菌（Porphyromonas gingivalis， P.gingivalis）诱导人肺上皮细胞A549 凋亡的影响。方法 以微量肉汤稀释法， 通过检测不同浓度大蒜素在不同时间点对 P. gingivalis 的抑制作用，测定大蒜素对P.gingivalis 的最低抑菌浓度（MIC）以及最低杀菌浓度（MBC）。分别于 12 h 及 24 h 时， 以四唑盐（MTT）比色法检测不同浓度（64、 96、 128 mg/L）大蒜素对 P.gingivalis 感染后的 A549 存活率的影响， 以流式细胞术FITC/PI 染色法检测 24 h 时， P.gingivalis 与大蒜素（128 mg/L）分别以及共同作用对 A549 细胞凋亡率的影响。结果 大蒜素对 P.gingivalis 的 MIC 为 64 mg/L， MBC 为 128 mg/L。MTT 检测结果显示， P.gingivalis 感染后， 随着大蒜素浓度的增加， 细胞存活率增加， 差异有统计学意义 （P < 0.05）。大蒜素 （128 mg/L） 组的存活率与细胞对照组比较差异无统计学意义， 表明大蒜素对 A549 无明显细胞毒性。流式细胞术检测结果显示， 各组细胞凋亡率由高到低为： P.gingivalis 组>P.gingivalis+大蒜素组>大蒜素组>细胞对照组， 差异有统计学意义（P < 0.01）， 大蒜素对 P.gingivalis 感染后 A549 的凋亡有明显抑制作用。结论 大蒜素可有效抑制 P.gingivalis 对肺上皮细胞的感染， 对于治疗牙周炎合并肺部感染有良好的应用前景。

关键词: 紫单胞菌, 龈, 大蒜辣素, 细胞凋亡, 微生物敏感性试验, A549 细胞

Abstract: Abstract： Objective To investigate the influence of different concentrations of allicin in apoptosis of A549 cells induced by porphyromonas gingivalis (P.gingivalis). Methods The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of allicin in inhibiting P.gingivalis were investigated by broth dilution methods. The tetrazolium salts (MTT) assay was used to detect the viability of A549 cells infected by P.gingivalis and treated with different concentrations of allicin (64 mg/L, 96 mg/L and 128 mg/L). The flow cytometry FITC/PI staining was used to detect apoptotic rates of A549 cells treated by P.gingivalis and/or allicin for 24 h. Results The values of MIC and MBC of allicin for inhibiting P.gingivalis were 64 mg/L and 128 mg/L respectively. MTT assay showed that the cell viability was significantly increased with the increased concentration of allicin in a concentration- dependent manner (P < 0.05). There was no significant difference in cell viability between allicin (128 mg/L) group and control group, which showed that allicin was no significant cytotoxicity in A549 cells. Flow cytometry assay showed that the apoptotic rates from high to low were P.gingivalis group > P. gingivalis + allicin group > allicin group > control group with significant differences (P < 0.01). The apoptosis of A549 cells induced by P.gingivalis was significantly inhibited by allicin (P < 0.01). Conclusion Allicin can inhibit P.gingivalis infection in lung epithelial cells. There is a good prospect in the application of allicin in the treatment of pulmonary infection in patients with periodontitis.

Key words: porphyromonas gingivalis, allicin, apoptosis, microbial sensitivity tests, A549 cells