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TGF-β、Smad信号通路蛋白及α-SMA在糖尿病肾病患者肾组织中的表达和意义

房勃龙,韩鸿玲   

  1. 天津医科大学总医院
  • 收稿日期:2013-03-28 修回日期:2013-07-12 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 房勃龙

The Role of Expression of TGF-βand Smad Signaling Pathway andα-SMA in Patients with Diabetic Nephropathy

FANG Bo long,HAN Hong ling   

  1. Department of Nephrology, General Hospital of Tianjin Medical University
  • Received:2013-03-28 Revised:2013-07-12 Published:2013-11-15 Online:2013-11-15
  • Contact: FANG Bo long

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摘要：

【摘要】 目的  检测转化生长因子（TGF）-β、Smad信号通路蛋白和α-平滑肌肌动蛋白（α-SMA）在糖尿病肾病（DN）患者肾活检组织中的表达，探讨DN的发病机制。 方法  收集DN肾组织标本28例（DN组）；非DM行肾切除的正常肾组织10例为对照组。应用免疫组化方法检测TGF-β1、TGF-β受体（R）Ⅰ、TGF-βRⅡ、Smad2/3、α-SMA在肾组织中的表达。  结果 （1）TGF-β1、TGF-βRⅠ、TGF-βRⅡ、Smad2/3在DN组和对照组中均有表达，前者高于后者，DN组早期光镜下肾组织未见明显异常时即有明显的高表达，而随着疾病的进展未见表达增高的现象。肾小球、肾小管出现纤维化后并不表达上述因子。（2）对照组的α-SMA仅表达于肾血管、肾小球和肾小管，而肾间质未见α-SMA表达，而DN组α-SMA在上述组织中均有表达。 结论  TGF-β及Smad信号转导通路参与了DN的发病，DN可能与免疫复合物性肾小球肾炎具有相似的发病机制。

关键词: 糖尿病肾病, 转化生长因子β, Smad2蛋白质, Smad3蛋白质, 肌动蛋白类, 信号传导, 活组织检查, 免疫组织化学

Abstract:

[Abstract]  Objective  To investigate the mechanism of the pathogenesis of diabetic nephropathy (DN) bydetecting
the expressions of transforming growth factor (TGF)-β, Smad proteins and α-smooth muscle actin (α-SMA) in kidney biopsyof patients with diabetic nephropathy (DN).   Methods  Twenty-eight patients with DN who underwent renal biopsy were col-lected as DN group. Ten subjects without DN who underwent nephrectomy were taken as control group. The expressions of TGF-β1, TGF-βRⅠ,TGF-βRⅡ,Smad2/3andα-SMA in renal tissues were detected by immunohistochemistry stain.  Results   (1)TGF-β1,TGF-βRⅠ,TGF-βRⅡ,and Smad2/3were expressed in the glomeruli and tubules of both DNgroup and control group, while the expressions of TGF-βand Smad proteins were significantly higher in DN group than those in control group. At the early stage of DN, TGF-βand Smad proteins were significantly expressed even though there was no remarkable lesions observed by light microscopy. There was no correlation between increased expression and the progression of DN. These proteins were not expressed after glomerulusand renal tubule fibrosis. (2) In control group, α-SMA was identified only in the vascular walls, glomerulus and renal tubules, while it was expressed in almost all parts of kidney in DN group.   Conclusion  TGF-βand Smad signals involved in the pathogenesis of DN, which may have similar pathogenesis with immune complex glomerulonephritis

Key words: diabetic nephropathies, transforming growth factor beta, Smad2 protein, Smad3 protein, actins, signal transduction, biopsy, 免疫组织化学