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骨髓基质细胞对移植肌腱在骨隧道内愈合作用的组织学研究

曹红彬1,辛景义2,梁军3,李秀兰4,于顺禄3   

  1. 1. 天津市天津医院
    2. 天津医院创伤科
    3. 天津医院
    4.
  • 收稿日期:2011-12-29 修回日期:2012-04-07 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 曹红彬

The histological study of Mesenchymal Stem Cells on tendon-bone healing in bone tunnel

  • Received:2011-12-29 Revised:2012-04-07 Published:2012-09-15 Online:2012-09-15

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摘要： [摘要] 目的 研究骨髓基质细胞对移植肌腱在骨隧道内愈合方式的影响 方法 采用兔膝关节半腱肌重建前十字韧带悬吊固定模型，对照组为假手术组而试验组在隧道内植入骨髓基质细胞。分别于手术后4周、8周和12周采取组织，采用HE、Masson染色，观察骨隧道和肌腱移植物间的界面组织学特殊变化，分析腱骨愈合信息。结果 4周对照组腱骨间有腱－骨分离,骨髓基质细胞组腱骨间充满结缔组织；8周骨髓基质细胞组形成Sharpey's纤维；12周时骨髓基质细胞组形成大量Sharpey's纤维，在移植肌腱和骨隧道之间有纤维软骨，矿化软骨形成；对照组12周时开始出现Sharpey's纤维。结论 骨髓基质细胞对移植肌腱在骨隧道内的愈合起明显的促进作用，可以缩短腱骨愈合时间。

关键词: 骨髓基质细胞, 移植肌腱, 骨隧道, 愈合

Abstract: [Abstract] Objective To research the effect of Mesenchymal Stem Cells（MSCs）on tendon-bone healing in bone tunnel . Methods Anterior cruciate ligament reconstruction with semitendonous tendon was done in knee joints of New Zealand rabbits by suspend fixation model. Mesenchymal Stem Cells were implanted in bone tunnel in experimental group and only operation in control group. Specimens were collected 4 weeks, 8 weeks and 12 weeks after surgery. Sections were stained with HE, MASSON . Special changes of interface tissue between bone tunnel and tendon graft were observed and informations of healing between tendon and bone tunnel were analyzed. Results In MSCs group abundant penetrating Sharpey fibers were appeared at 8weeks and Sharpey fibers were appeared at 12 weeks in control group，direct connection can be seen in MSCs group. Conclusion MSCs can accelerate tendon healing in bone tunnel.

Key words: Mesenchymal Stem Cells, Tendon graft, Bone tunnel, Healing