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通经补肾复方对阿尔茨海默病模型大鼠氧化应激的影响

程书珍1,王丽君2,刘恒3,王晓梅1,刘爱民4,骆朋好5   

  1. 1. 河北工程大学医学院
    2. 邯郸长征医院
    3. 河北工程大学文学院
    4. 邯郸市中心医院
    5. 大名县妇幼保健院
  • 收稿日期:2011-09-28 修回日期:2011-12-15 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 程书珍

Effect of Tongjing Bushen Recipe on Oxidative Stress of Alzheimer’s Disease Model Rats

  • Received:2011-09-28 Revised:2011-12-15 Published:2012-09-15 Online:2012-09-15

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摘要： 摘要 目的: 观察通经补肾中药方对铝诱导的阿尔茨海默病(Alzheimer’s disease， AD)模型大鼠氧化应激(oxidative stress, OS)的影响。方法: 选择健康3月龄SD大鼠84只，随机分为对照组（C组）、模型组（按剂量从小到大分为M1、M2、M3组）、中药组（按剂量从小到大分为N1、N2、N3组），每组各12只。模型组在常规饲料中添加不同剂量 A1C13?6H2O喂饲大鼠，持续染毒3个月，制做AD动物模型。用电迷宫测试大鼠学习记忆能力，模型组大鼠对电刺激的逃避潜伏期明显延长，逃避正确率显著降低，证明AD模型制备成功。中药组大鼠喂饲高剂量铝饲料，并以不同剂量通经补肾中药方灌胃。上述实验结束后，检测大鼠脑组织及血清SOD、GSH-Px活性和MDA水平。结果: 与C组比较，M2、M3组大鼠脑组织及血清中SOD、GSH-Px的活性均明显降低(P<0.01)，MDA 含量显著增高(P<0.01)。 M1组与C组比较，M2组与M3组比较，各指标均无显著性差异(P>0.05)。与M3组比较，N2、N3组大鼠SOD、GSH-Px的活性明显增高(P<0.01)，MDA含量明显降低(P<0.01)。N1组与M3组比较，N2、N3组比较，各指标均无显著性差异 (P>0.05)。结论: 铝的过多摄入使大鼠处于强氧化应激状态，通经补肾中药方可显著提高模型大鼠抗氧化能力，从而对中枢神经系统有保护作用。胃。上述实验结束后，麻醉大鼠，打开胸腔，右心室采血，分离血清保存,同时快速取大脑做脑匀浆,检测脑组织及血清SOD、GSH-Px活性和MDA水平。结果 与对照组比较，中、高剂量铝饲料组大鼠脑组织及血清中SOD、GSH-Px的活性均明显降低(P<0.01)，MDA含量显著增高(P<0.01)。与高剂量铝饲料组比较，中、高剂量中药组大鼠SOD、GSH-Px的活性明显增高(P<0.01)，MDA含量明显降低(P<0.01)。结论 铝的过多摄入使大鼠处于强氧化应激(oxidative stress,OS)状态，通经补肾中药方可显著提高模型大鼠抗氧化能力，从而对中枢神经系统有保护作用。

关键词: 通经补肾复方, 铝, 阿尔茨海默病, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 丙二醛

Abstract: Abstract Objective: Observe the effect of Tongjing Bushen recipe on oxidative stress of Alzheimer’s Disease model rats induced by Al. Methods: Chose 84 healthy SD rats, 3-month old, randomly divided them into Control Group(Group C), Model Group ( including low-dosed Al feed group—Group M1, middle-dosed Al feed group—Group M2 and high- dosed Al feed group—Group M3) and Chinese-medicine-recipe Group (including low-dosed group—Group N1, middle-dosed group—Group N2, high- dosed group—Group N3).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. Determine the learning and memory ability of rats with Y-electric. The escaping latent periods of rats in Group M were obviously prolonged, the escaping correct rates of rats in Group M obviously descended. The AD animal model was proved to be successful. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were taken blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Group C, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of Group M2 and Group M3 were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared the rats in Group M1 with the rats in Group C and also compared the rats in Group M2 with the rats in Group M3, each index had no striking difference (P>0.05).Compared with the rats in Group M3, the activities of SOD and GSH-PX of the rats Group N2 and Group N3 strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01). Compared the rats in Group M3 with the rats in Group N1 and also compared the rats in Group N2 with the rats in Group N3, each index had no striking difference (P>0.05). Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system.group, middle-dosed Al feed group and high- dosed Al feed group) and Chinese-medicine-recipe Group (including low-dosed group, middle-dosed group, high- dosed group).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were anesthetized, open their chests, took blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Control Group, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of middle- and high- dosed Al feed group were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared with the rats in high-dosed Al feed groups, the activities of SOD and GSH-PX of the rats in model- and high- dosed Chinese medicine groups strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01) Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system.

Key words: Tongjing Bushen Recipe, aluminum, Alzheimer’s disease, super oxide dismutase, glutathione peroxidase, malonaldehyde