天津医药

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手足口病患儿外周血淋巴细胞亚群及血清IL-6、IL-10变化的研究

王经伟   

  1. 山东省临沂市人民医院 感染科
  • 收稿日期:2011-10-31 修回日期:2012-03-21 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 王经伟

The study of peripheral blood lymphocyte subsets and serum IL-6, IL-10 in children with hand-foot-mouth diseases

  • Received:2011-10-31 Revised:2012-03-21 Published:2012-09-15 Online:2012-09-15

摘要： 【摘要】 目的 研究手足口病患儿外周血淋巴细胞亚群及血浆IL-6、IL-10水平的变化，以探讨其在手足口病发病机制中的作用。 方法 应用流式细胞仪检测82例手足口病患儿外周血及30例健康同龄儿童外周血的淋巴细胞亚群CD3+、CD4+、CD8+、NK及B淋巴细胞比例；应用双抗体夹心ELISA法检测其血清IL-6、IL-10水平。结果 手足口病患儿普通组与对照组比较： CD3+、CD4+淋巴细胞比例明显降低，差异显著（p<0.01），CD8+、NK细胞比例及CD4+/CD8+比值变化不明显（p>0.05），B淋巴细胞比例明显升高，差异显著（p<0.01），血清IL-6、IL-10变化不明显（p>0.05）；手足口病患儿重症组与对照组比较： CD3+、CD4+淋巴细胞比例及CD4+/CD8+比值明显降低，差异显著（p<0.01），CD8+、NK细胞比例变化不明显（p>0.05），B淋巴细胞比例明显升高，差异显著（p<0.01），血清IL-6、IL-10水平明显升高，差异显著（p<0.01）；手足口病患儿重症组与普通组比较：和重症组与对照组比较的结果相似。结论 手足口病患儿表现为T淋巴细胞不同程度相对抑制、B淋巴细胞呈增殖反应及细胞因子过度分泌，提示手足口病患儿尤其重症患儿存在显著的免疫功能紊乱。

关键词: 手足口病, 淋巴细胞亚群, 白细胞介素6(IL-6), 白细胞介素10(IL-10), 流式细胞术

Abstract: 【Abstract】Objective To explore the pathogenesis of hand-foot-mouth disease(HFMD)，the changes of lymphocyte T subsets and the level of serum interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined. Methods The changes of lymphocyte T subsets(CD3+、CD4+、CD8+、NK、CD4+/CD8+) and the level of serum IL-6，IL-10 were assayed in 82 patients of HFMD and 30 normal controls respectively by flow cytometry and by enzyme-linked immuno sorbent assay(ELISA). Results Simple cases of HFMD compared with control group: The percentages of lymphocyte CD3+,CD4+ were significantly reduced（p<0.01）, and no significant difference was observed in the percentages of CD8+, natural killer(NK)cells and the ratio of CD4+/CD8+ between simple patients and controls(P>0.05). The ratio of lymphocyte B showed significant increases（p<0.01）, while serum IL-6，IL-10 were detected no significant difference among simple cases and controls（p>0.05）; Severe cases of HFMD compared with control group: The percentages of lymphocyte CD3+,CD4+ and the ratio of CD4+/CD8+ were significantly reduced（p<0.01）, and no significant difference was observed in the percentages of CD8+ and NK cells between severe cases and controls（p>0.05）. The ratio of lymphocyte B and serum IL-6, IL-10 showed significant increases（p<0.01）. The results of severe cases compared with simple cases were similar to compared with controls. Conclution Lymphocyte T in children with HFMD appearred relatively inhibition in varying degrees, lymphocyte B proliferation in response ,and cytokines excessively secretion. The above indicated that the immunity of children with HFMD, especially severe cases, existed dysfunction obviously.

Key words: Hand-foot-mouth disease, lymphocyte subsets, Interleukin-6(IL-6), Interleukin-10(IL-10), Flow cytometry