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磷酸肌酸钠预给药对大鼠局灶性脑缺血再灌注损伤的影响

李波   

  1. 天津医科大学第二医院
  • 收稿日期:2010-10-18 修回日期:2011-01-17 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 李波

The effect of exogenous creatine phosphate Pre-conditioning on rat’s focal cerebral ischemia/reperfusion injury on rats in Vivo

  • Received:2010-10-18 Revised:2011-01-17 Published:2011-09-15 Online:2011-09-15

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摘要： 摘要 目的：观察不同浓度磷酸肌酸钠预给药对大脑中动脉线栓法（MCAO）致大鼠脑缺血/再灌注损伤模型脑梗死体积的影响。方法 采用MCAO法建立大鼠局灶性脑缺血/再灌注损伤模型。将50只SD大鼠，随机分为假手术组（Shame组）、缺血/再灌注损伤组(IR组) 、低浓度磷酸肌酸钠预给药组（L组）、中浓度肌酸磷酸钠预给药组（M组）、高浓度磷酸肌酸钠预给药组（H组），每组10只。再灌注末，测脑组织髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、丙二醛（MDA）、Na+-K+-ATP酶含量变化。实验结束对脑组织进行HE染色、TTC染色等病理技术手段处理，观察脑组织损伤情况。结果 再灌注24h末，（1）Shame组脑组织酶学变化与其它四组比较，差异有统计学意义(P<0.05）；（2）MPO、MDA活性变化， IR组与L组、M组、H组比较明显升高， M组与H组相比较，M组血清酶学活性明显升高，差异均有统计学意(P<0.05）； （3）SOD、Na+-K+-ATP酶活性变化，IR组与L组、M组、H组比较明显降低， M组与H组相比较，M组血清酶学活性明显降低，差异均有统计学意(P<0.05）；差异有统计学意义(P<0.05）；（4）Bederson神经功能缺损评分表明Shame组无损伤，IR组、H、M、L组均出现不同程度的神经损伤。（5）脑梗死体积百分比，Shame组脑组织无梗死，IR组脑梗死体积百分比较其它三组明显增大；L组脑梗死体积百分比大于M组、H组；M组脑梗死体积大于H组，差异均有统计学意义 (P<0.05）。 结论 磷酸肌酸钠预给药可有效地减少局灶性脑缺血再灌注损伤后大鼠脑梗死体积，对脑缺血再灌注损伤后的有减轻作用。

关键词: 磷酸肌酸钠, 脑, 梗死体积 , 缺血/再灌注损伤

Abstract: Abstract Objective： To observe the effect of creatine phosphate on cerebral infarction volume in focal cerebral ischemia/reperfusion rat model．Methods: Rats model of focal cerebral ischemia/reperfusion injury were randomly divided into 5 groups：groupⅠ,shame operation, groupⅡ,ischemia/reperfusion injury (I/R); groupⅢ,low dosage of creatine phosphate 20mg/kg（L）; groupⅣ, medium dosage of creatine phosphate 40mg/kg (M); groupⅤ,high dosage 80mg/kg（H）; The activity of SOD、MPO 、MDA and Na+-K+-ATP enzyme were measured at the end of reperfusion. Infarction conditions and volume of cerebral was determined by dyeing with hematoxylin eosin (HE) and triphenyltetrazolium staining at the end of reperfusion. Results ：As far as the activity of MDA、SOD、MPO and Na+-K+-ATP enzyme, there are great significant differences among five groups at the end of reperfusion（P<0.05）. There is also great significant difference of infarction volume between group Ⅲ and groupⅣ（P<0.05）.Conclusion: pre-conditioning of creatine phosphate can attenuate ischemia/reperfusion injury of focal cerebral.

Key words: creatine phosphate sodium, cerebral, infarction volume , ischemia /reperfusion injury