天津医药

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CD4+IL-10+调节性T细胞在特应性皮炎患者中的改变

赵宏丽,赵俊芳,李孟娟   

  1. 天津市中医药研究院附属医院
  • 收稿日期:2011-08-04 修回日期:2011-11-25 出版日期:2012-08-15 发布日期:2012-08-15
  • 通讯作者: 赵宏丽

Change of CD4+IL-10+regulatory T Cell in Atopic Dermatitis patients

  • Received:2011-08-04 Revised:2011-11-25 Published:2012-08-15 Online:2012-08-15
  • Contact: Hong-Li ZHAO

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摘要： 摘要 目的 探讨特应性皮炎患者CD4+IL-10+调节性T细胞及其细胞因子的改变。方法 用流氏细胞仪直接免疫荧光法检测AD患者外周血CD4+IL-10+T细胞的绝对计数和百分率；用酶联免疫吸附试验检测外周血细胞因子IL-10、TGF-β的浓度。 结果AD患者CD4+IL-10+T细胞绝对计数为0.26+0.126×109/L，占CD4+T细胞的百分率是12.76+4.85%；而对照组CD4+IL-10+T细胞绝对计数为0.016+0.007×109/L，占CD4+T细胞的百分率是3.27+1.03%。经t检验，AD患者CD4+IL-10+T细胞绝对计数和百分率均高于对照组，有统计学差异（p <0.05）。 AD患者外周血IL-10浓度为126.59+59.46 pg/ml，高于正常对照组（31.47+10.96 pg/ml），经t检验，有统计学差异（p<0.05）。AD患者外周血TGF-β浓度为407.23+155.88 pg/ml，高于正常对照组（359.47+99.21 pg/ml），但经t检验，没有统计学差异（p>0.05）。CD4+IL-10+T细胞绝对计数、百分率均与外周血IL-10浓度呈正相关，有统计学意义（r=0.47，r=0.65, p <0.05）；而与外周血TGF-β无统计学相关性（r=0.15，r=0.21 , p >0.05）。结论 IL-10可能是Trl发挥功能的主要因子，通过下调由Thl和Th2细胞介导的炎症反应过程，参与免疫调节。

关键词: 皮炎, 特应性, T淋巴细胞, 调节性, 白细胞介素10, 转化生长因子β

Abstract: Abstract: Objective To explore the change of CD4 + IL-10 + regulatory T cells and cytokine in atopic dermatitis. Methods The direct immunofluorescence assay was used to detect CD4+ IL-10 +Tcell by flow cytometry. ELISA was used to assay IL-10 and TGF-βin peripheral blood. Results The absolute count of CD4 + IL-10 + T cells is 0.26 + 0.126×109 / L, and the percentage is 12.76 + 4.85% in AD patients . They are higher than in control group(0.016 + 0.007×109 / L; 3.27 + 1.03%). There ere statistically significant (p < 0.05). The peripheral blood IL-10 concentration in AD patients is 126.59+ 59.46 pg/ml, higher than normal control group (31.47 +10.96 pg/ml) (p < 0.05). The peripheral blood TGF-βconcentration in AD patients is 407.23 + 155.88 pg/ml, higher than normal control group (359.47 + 99.21 pg/ml) (p > 0.05). There are statistically positive correlation between absolute and percentage of CD4 + IL-10 + T cell and IL-10 concentration (r = 0.47, r = 0.65, p < 0.05). Whereas There are no statistically correlation between absolute and percentage of CD4 + IL-10 + T cell and TGF-βconcentration (r = 0.15, r = 0.21, p > 0.05). Conclusion IL-10 is the main functional f actor of Trl. It can involved in immune adjustment by cutting inflammation process mediated by Thl and Th2 cell.

Key words: Dermatitis, Atopic(AD), T cell, regulatory, IL-10, TGF-β