天津医药

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459例女性健康体检者HPV基因型及高危因素分析

陈秀杰1,李艳玲2,曲芃芃2   

  1. 1. 天津医科大学研究生院
    2. 天津市中心妇产科医院
  • 收稿日期:2013-07-30 修回日期:2013-10-23 出版日期:2014-02-15 发布日期:2014-02-15
  • 通讯作者: 曲芃芃

The Analysis of HPV Genotypes and Related High Risk Factors in 459 Women Conducted Health Examination

  • Received:2013-07-30 Revised:2013-10-23 Published:2014-02-15 Online:2014-02-15

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摘要： 目的 研究459例女性健康体检者宫颈人乳头瘤病毒（HPV）感染情况、基因型别分布及其高危因素。方法 采用巢式PCR和焦磷酸测序技术检测459例受检者宫颈脱落细胞中HPV分型情况，同时收集受检者一般资料、月经婚育史、生殖道疾病既往史，分析HPV感染的相关危险因素。结果 HPV阳性检出率为17.9%，共检测出7种高危型别，其中HPV16检出率最高（9.8%），其次是HPV58（7.0%），HPV18（5.2%）。单纯感染、双重感染、多重感染的检出率分别是9.6%、4.8%、1.5%。饮酒、经济收入﹤3000元/月、性伴侣﹥1个、性生活频率﹥4次/月、伴有宫颈糜烂是HPV 感染的危险因素（P＜0.05）。结论 HPV DNA基因型别检测可为宫颈癌的早期筛查和HPV疫苗的研发使用提供重要信息，为高危人群的早预防、早诊断、早治疗提供重要依据。

关键词: 乳头状瘤病毒科, 宫颈肿瘤, 癌, 基因型, 危险因素, 聚合酶链反应, 焦磷酸测序技术

Abstract: Objective To investigate the HPV infection, genotypes and related high risk factors among 459 women conducted health examination. Methods 459 cervical cytology specimens were detected with nest PCR and pyrosequencing，questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed . Results 82 of 465 cases (17.9%) were infected with HPV. 7 kinds of HR-HPV were found. HPV 16 were the most common (9.8%), HPV58 (7.0%), HPV18 (5.2%) orderly. The rate of single, duplex, multiplex infection was 9.6%, 4.8%, 1.5% respectively. HPV infection was closely correlated with drinking，low economy condition，having multiple sexual partners，sexually active and cervical erosion (P＜0.05). Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines; it also contributes to provide significant evidence for the prevention, diagnosis, and treatment of cervical cancer at early stage for high risk population.

Key words: papillomaviridae, uterine cervical neoplasms, carcinoma, genotype, risk factors, polymerase chain reaction, pyrosequencing