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黄芪注射液对阿霉素中毒性心肌损伤小鼠心肌细胞线粒体膜电位及GSH的影响

梁丽英1,陈晶1,2,贺毅1,2   

  1. 1. 广西中医学院第一附属医院
    2.
  • 收稿日期:2010-09-14 修回日期:2011-01-20 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 梁丽英

Effect of the Huangqi Injection on the Mitochondrial Membrane Potential (MMP) and GSH in ADR Mice

  • Received:2010-09-14 Revised:2011-01-20 Published:2011-07-15 Online:2011-07-15

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摘要： 摘要 目的：观察黄芪注射液对ADR小鼠心肌线粒体膜电位（△Ψm)及GSH的影响。方法：取昆明小鼠60只，体质量为18～22 g，随机分为黄芪注射液治疗组（大、中、小3个剂量组）、阿霉素模型组和正常对照组，建立阿霉素中毒性心肌损伤模型(ADR)，实验结束24 h后处死小鼠，测定心脏系数和心肌组织中还原型谷胱甘肽（GSH）的含量；应用流式细胞仪分析技术 (flowcytometry, FCM) 检测小鼠心肌细胞线粒体膜电位。结果：1. 心脏系数 黄芪注射液各剂量组分别与阿霉素模型组比较，均有显著性降低（P<0.01）；2. GSH含量 黄芪注射液各剂量组分别与阿霉素模系型组比较均有显著性增高（P<0.01）；黄芪注射液剂量组相互比较，还原型谷胱甘肽含量虽然数值上有差别，但无统计学意义（P>0.05）。3. 线粒体膜电位 黄芪注射液各剂量组分别与阿霉素模型组比较明显增高（P<0.01），黄芪注射液各剂量组与正常对照组比较，也有差异（P<0.05），结论：黄芪注射液可通过减轻小鼠心脏肿大，提高心肌内GSH含量，稳定线粒体膜电位，抑制心肌细胞发生异常凋亡来实现其保护心肌细胞的作用。

关键词: 黄芪注射液, 阿霉素, 线粒体膜电位, GSH

Abstract: Abstract: Objective To observe the Effect of the Huangqi Injection on the Mitochondrial Membrane Potential (MMP) and GSH in ADR mice. Methods 60 Kunming mice (weighing 18～22g) are randomly divided into five groups:they are the normal control group ,the adriamycin (ADR) group,the Huangqi injection (large、middle、small) group ,instruct the molder of the doxorubicin induced cardiotoxicity in mice.Test the coefficient of the heart,the contentof the GSH. Flow Cytometry are used to analysis in Mitochondrial Membrane Potential in this study. Results 1. heart coefficient: Compared with the adriamycin(ADR) group, Huangqi injection groups can relieve the heart of tumefy(P<0.01); 2.the content of GSH: Compared with the adriamycin(ADR) group, Huangqi injection groups can increase the GSH(p<0.01) .3. the MMP: Huangqi injection groups can stable the cardio-muscle cell mitochondrial membrane potential, Compared with the adriamycin(ADR) group，there are obvious statistical differences (P<0.01). Conclusion:Huangqi injection can relieve the heart of tumefy,increase the GSH, stable the cardio-muscle cell mitochondrial membrane potential，restrain the apoptosis cardiac muscle cell to protect cardiac muscle cell.

Key words: Huangqi Injection, Adriamycin, MMP, GSH