天津医药

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雌激素对实验性自身免疫性脑脊髓炎小鼠治疗作用的观察

胡晓,王建怡   

  1. 贵州省人民医院
  • 收稿日期:2010-11-01 修回日期:2011-02-18 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 王建怡

The Therapeutical Effect of Estrogen on Experimental autoimmune encephalomyelitis mice

Xiao Hu1,   

  • Received:2010-11-01 Revised:2011-02-18 Published:2011-07-15 Online:2011-07-15

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摘要： 目的 观察雌激素对实验性自身免疫性脑脊髓炎（EAE）小鼠中枢神经系统（CNS）髓鞘及轴突损伤的影响。方法 用MOG35-55多肽诱发40只EAE小鼠模型，做去卵巢术。分为治疗组和对照组，治疗组予雌激素治疗。比较两组EAE小鼠的临床症状评分。取脑和脊髓，行Luxol Fast Blue（LFB）-H&E染色及Bielschowsky染色观察各组EAE小鼠髓鞘及轴突损伤情况；实时荧光定量PCR及Western-blot检测各组EAE小鼠CNS中（髓鞘碱性蛋白）MBP及生长相关蛋白-43（GAP-43）的表达。结果 治疗组EAE小鼠与对照组相比临床症状减轻、发病率降低；LFB-H&E染色，Bielschowsky染色，实时荧光定量PCR及Western-blot结果显示治疗组髓鞘及轴突损伤减轻。结论 雌激素通过减轻髓鞘损伤及促进轴突再生治疗EAE小鼠。

关键词: 实验性自身免疫性脑脊髓炎, 雌激素, 髓鞘损伤, 轴突再生, 多发性硬化

Abstract: Objective To Observe the effect of estrogen on myelin and axonal injury in the central nervous system(CNS) of experimental autoimmune encephalomyelitis（EAE） mice. Methods 40 mice EAE models were induced with MOG35-55 peptide, all animal ovarectomized. They were divided into treatment group and control group. Treatment group was treated by estrogen. Two groups were compared by clinical symptom scores. The myelin and axonal injury of the brain and spinal cord were studied by Luxol Fast Blue（LFB）-H&E and Bielschowsky staining. The expression of MBP and GAP-43 were examined by quantitative real-time PCR and western-blot. Results Clinical symptom score and incidence of treatment group were lower than that in the control group. Luxol Fast Blue（LFB）-H&E, Bielschowsky staining, quantitative real-time PCR and western-blot showed that myelin and axonal injury was decreased in treatment group. Conclusion Estrogen could decrease myelin damage and promote axonal regeneration to treat EAE mice.

Key words: experimental autoimmune encephalomyelitis, estrogen, myelin damage, axonal regeneration, multiple sclerosis