天津医药

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蒿鳖养阴软坚方对ConA诱导小鼠肝纤维化的预防作用

刘红艳   

  1. 天津市南开医院
  • 收稿日期:2010-09-15 修回日期:2011-05-04 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 刘红艳

The Preventive Effect of HaoBieYangYinRuanJian on Hepatic Fibrosis Induced by Concanavalin A

  • Received:2010-09-15 Revised:2011-05-04 Published:2011-07-15 Online:2011-07-15

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摘要： 摘要 目的：探讨蒿鳖养阴软坚方对ConA诱导小鼠肝纤维化的预防作用。方法：小鼠94只按体质量随机区组分为7组。正常组8只，模型组16只，蒿鳖养阴软坚方高剂量组14只，中剂量组14只，低剂量组14只，复方鳖甲软肝片组14只和秋水仙碱组14只。采用尾静脉注射ConA制备小鼠肝纤维化模型，以蒿鳖养阴软坚方预防用药，HE染色及胶原染色观察肝组织形态，盐酸水解法测定肝组织胶原蛋白的含量，放射免疫法测定血清透明质酸(HA)和Ⅲ型前胶原(PCⅢ)水平。结果：模型组肝纤维化程度分期及计分分别显著高于正常组(P<0.01)，高、中、低剂量组皆明显低于模型组(P<0.05)；模型组肝组织胶原蛋白含量显著高于正常组(P<0.01)，高、中、低剂量组皆明显低于模型组(P<0.05)；该方能一定程度的降低血清HA及PCⅢ水平(P>0.05)。结论：蒿鳖养阴软坚方对ConA诱导小鼠肝纤维化具有预防作用。 关键词： ConA 肝纤维化 胶原蛋白 透明质酸 Ⅲ型前胶原 小鼠，Balb/c

关键词: ConA, 肝纤维化, 胶原蛋白, 透明质酸, Ⅲ型前胶原, 小鼠, Balb/c

Abstract: Objective: To investigate HBYYRJ prophylactic effect on mouse liver fibrosis by reduced by ConA. Methods: Eighty rats were randomly divided into 7 groups, normal group(n=8), model group(n=16), HBYYBJ low dosage treatment group(n=14), middle dosage treatment group(n=14), high dosage treatment group(n=14), composed formula treatment group(n=14), and colchicine treatment group(n=14). Rat models of hepatic fibrosis were established by injection of ConcanavalinA .HBYYBJ was used for prevention of hepatic fibrosis. Histologic observation of the liver tissue was done with H & E stain and collagen stain.。Result: The degree of the liver fibrosis staging are significantly higher than normal contrast group (P<0.01). The three groups of HBYYRJ, the degree of hepatic fibrosis are significantly lower than model group (P<0.05).The degree of liver fibrosis The content of HBYYRJ in model group was significantly higher than normal contrast group (P<0.01) HBYYRJ medium and low groups was significantly lower than model contrast (P<0.05). HBYYRJ can decreace the degree of HA and PCⅢ. Conclusion: HBYYRJ has the preventive effect on the liver fibrosis with mice. Key words: ConA, liver fibrosis, hydroxyproline (Hyp), hyaluronic acid (HA), pre--collagenⅢ(PCIII)

Key words: ConA, liver fibrosis, hydroxyproline (Hyp), hyaluronic acid (HA), pre--collagenⅢ(PCIII), Balb/c