天津医药

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脂氧素对子痫前期大鼠模型的治疗的研究

张俊农   

  1. 天津市医科大学第二医院
  • 收稿日期:2011-12-01 修回日期:2012-08-22 出版日期:2013-03-15 发布日期:2013-03-15
  • 通讯作者: 张俊农

Research of the treatment of lipoxin A4 to preeclampsia rats model

ZHANG Jun nong   

  1. Department of Obstetrics and Gynecology，The Second Affiliated Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2011-12-01 Revised:2012-08-22 Published:2013-03-15 Online:2013-03-15
  • Contact: ZHANG Jun nong

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摘要：

【摘要】目的 探讨脂氧素（LX）对子日前期大鼠血清NO浓度的影响，为其治疗妊娠高血压疾患奠定基础。方法 随机将30只妊娠大鼠分为正常组（生理盐水）、内毒素组（内毒素）和LX组（内毒素+LXA4），每组10只。小剂量内毒素建立子日前期大鼠模型，LX组于注入内毒素当天，尾静脉注LXA4注射液，正常组和内毒素组大鼠尾静脉注射等量的生理盐水，确定模型建立成功后，于孕前、妊娠第8、15、18天测量孕鼠血压、蛋白尿和LX水平，孕第20天手术取出子鼠，测量新生鼠体质量，同时采用硝酸还原酶法测定孕鼠腹主动脉血清NO浓度。双抗体夹心法测定大鼠LX 水平。结果 大鼠内源性脂氧素水平随孕周有所升高，孕第18天内毒素组大鼠脂氧素水平较正常组有所下降。LX 组子鼠体质量及NO浓度高于内毒素组，血压及尿蛋白含量低于内毒素组，差异有统计学意义(P < 0.05)。结论 脂氧素A4对子日前期大鼠模型有治疗作用。

关键词: 脂氧素, 子痫前期, 一氧化氮, 大鼠

Abstract:

[Abstract] Objective  To investigate the effects of lipoxin (LX) on the plasma nitric oxide (NO) concentration in preeclampsia rats, and make an experiment foundation for the treatment of human preeclampsia. Methods Thirty pregnant rats were randomly divided into three groups: normal group (saline), preeclampsia group (endotoxin) and LX group (endotoxin + LX). The experimental rat model of preeclampsia was established with small dose of endotoxin. LX was tail injected intravenously in the day that endotoxin was injected in LX group. The same volume of normal saline was tail injected intravenously in saline and endotoxin groups. Levels of blood pressure, urine protein and LX were detected on 8, 15 and 18 days after successful Modeling. The body weights of newborn rats were measured after operation on the 20th day of pregnancy. The NO concentration of abdominal aorta in mother rats was detected by nitrate reductase enzyme method. The level of LX was detected by double antibody sandwich method. Results  The endogenous lipoxin level was increased with the gestational age. The level of LX on 8 days of pregnancy was decreased in endotoxin group than that of saline group. The body weights of newborn rats and NO levels of pregnant rats were significantly higher in LX group than those of endotoxin group. There were significantly lower levels of blood pressure and urine protein in LX group than those of endotoxin group (P < 0.05). Conclusion  Lipoxin may be potentially useful in treatment of preeclampsia in rats．

Key words: Lipoxin A4, Experimental preeclampsia, Nitrogen monoxide, Rat