天津医药

• 实验研究 •    

神经生长因子对胰淀素所致大鼠胰岛细胞功能障碍的保护作用

崔巧丽1,隋志芳2,王银霞2,朱铁虹1   

  1. 1. 天津医科大学总医院
    2.
  • 收稿日期:2009-12-31 修回日期:2010-01-26 出版日期:2010-05-15 发布日期:2010-05-15
  • 通讯作者: 崔巧丽

Effect of NGF in protecting rat islet cells from Amylin-induced dysfunction.

  • Received:2009-12-31 Revised:2010-01-26 Published:2010-05-15 Online:2010-05-15

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摘要： 摘要 目的： 观察神经生长因子(NGF)对高浓度胰淀素作用下胰岛细胞活力和功能的影响。方法：分别以不同浓度的NGF孵育胰淀素作用过的胰岛细胞，比较各组细胞对MTT还原能力和胰岛素的分泌能力。结果：高浓度胰淀素作用的胰岛细胞对MTT还原能力和胰岛素分泌量与对照组相比均明显下降（p<0.01）,差异具有统计学意义。在NGF作用组，NGF50ng/mL与对照组相比差异没有统计学意义。从NGF100 ng/mL开始胰淀素作用的胰岛细胞对MTT还原能力逐步增强、基础状态和葡萄糖刺激下胰岛素的分泌量也显著增加，差异均具有统计学意义。（p<0.05）并具有剂量依赖性。结论：NGF可减轻高浓度胰淀素对胰岛细胞活力的抑制作用，并促进胰岛B细胞分泌功能的恢复。

关键词: 神经生长因子, 胰淀素, 胰岛B细胞, 胰岛素

Abstract: Abstract Objective:To observe the influence of nerve growth factors (NGF) on vitality and function of the islet cell by the action of Amylin. Methods:Islet cells under the Amylin were incubated with NGF in different concentration ,and we observed the MTT reduction capacity and the amount of insulin secretion.The latter contains the level of insulin both base and high glucose condition .Results: Remarkable decrease both in the secretion of insulin and the reduction capacity on MTT was noticed in the 10umol/lAmylin group. And when were incubated with NGF(>50ng/ml) the inhibition can be dose-dependent relieved. Conclusion: NGF can restore Islet cell’s viability inhibited by high concentration amylin,and recovery the secretory functional of islet B cell.

Key words: Nerve Growing Factor (NGF), Amylin, Islet B cell, Insulin