天津医药

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普伐他汀对大鼠急性心肌梗死后热休克蛋白B7表达的影响

蒋友旭1,张丽华2   

  1. 1. 郑州大学第二附属医院
    2. 郑州大学第二附属医院心内一科
  • 收稿日期:2013-05-06 修回日期:2013-09-27 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 张丽华

The effect of Pravastatin on the Expression of HSPB7 in acute myocardial infarction rats

  • Received:2013-05-06 Revised:2013-09-27 Published:2014-03-15 Online:2014-03-15

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摘要： 目的 观察急性心肌梗死(AMI)大鼠梗死区心肌组织中热休克蛋白B7（heat shock protein B7,HSPB7）mRNA 和蛋白的表达及普伐他汀的干预作用。方法 80只AMI大鼠随机分为心肌梗死组（AMI）和普伐他汀组（P），每组40只，同时设假手术组（SH）大鼠40只，再将3组大鼠分为1h组、3h组、6h组和12h组，每个时间组10只。SH组开胸后不结扎冠脉，AMI组开胸后结扎冠状左前降支，P组开胸后结扎冠状左前降支，同时给予普伐他汀0.5mg/（kg?d）灌胃（其余组给予等量蒸馏水灌胃）。分别于各时间点处死大鼠，取左心室梗死区心肌组织，假手术组取对应部位的心肌组织，分别采用RT-PCR 和免疫组化法检测HSPB7 mRNA 和蛋白的表达。结果: 各AMI组和P组大鼠梗死区心肌组织HSPB7 mRNA 和蛋白与SH组的表达相比，差异均有统计学意义(P＜0.001);梗死后1h，梗死区心肌HSPB7 mRNA 和蛋白的表达增加( P＜0.05)，在3h时达到高峰，6h组 、12h组mRNA 和蛋白的表达仍高于假手术组，且P组中各时间点HSPB7的表达比AMI组高。结论: HSPB7能够在AMI早期表达，普伐他汀可促进AMI后梗死区心肌组织HSPB7表达，可能是其在心梗早期起到保护心肌作用的机制之一

关键词: 急性心肌梗死, 热休克蛋白B7, 普伐他汀

Abstract: Objective To observe The effect of Pravastatin on the Expression of HSPB7 in acute myocardial infarction rats. Methods The 80 AMI rats were randomly divided into acute myocardial infarction group(AMI), and Pravastatin group(P), the 40 SD rat2 in sham operation group(SH),then subdivide each group into group 1h ,3h ,6h and 12h. SH was not ligated but threaded; AMI was created by ligation of the 1eft anterior descending coronary artery; Group P created by ligation of the 1eft anterior descending coronary artery with a Pravastatin of 0.5mg/（kg?d），the other groups were given the same amount normal saline via gavage. The myocardial tissue from left ventricular infarcted border zone was harvested at each time interval，while the corresponding myocardial tissue in sham-operated rats was harvested．HSPB7 mRNA and protein expressions were measured by RT-PCR and immunohistochemistry respectively．Results The expressions of HSPB7 mRNA and protein in the AMI groups and P groups were statistically significant( P＜0.001). All the above indexes exhibited an ascending tendency at 1h after AMI( P＜0.05)，reached peak value at 3h after AMI，and remaining detectable up to 12h after AMI，The P groups were still higher than those in the sham operation group. Conclusion HSPB7 could express in acute myocardial infarction rats in early times, and Pravastatin could promote the upregulation of the expressions of HSPB7 in myocardial infarcted border zone after AMI，which may be one of the mechanisms to protect myocardial after AMI

Key words: Acute myocardial infarction, HSPB7, Pravastatin