天津医药

• 细胞与分子生物学 • 上一篇    下一篇

反义miR-30d对乳腺癌细胞侵袭迁移能力的影响

陈戈1,骆萍1,龚宇1,周瑶1,赵瑞君1,谢春伟2   

  1. 1. 南昌市第三医院乳腺外科
    2. 江西省南昌市象山南路2号南昌市第三医院
  • 收稿日期:2013-05-06 修回日期:2013-10-16 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 谢春伟

The effect of antisense miR-30d on invasion and migration in breast cancer cells

  • Received:2013-05-06 Revised:2013-10-16 Published:2014-03-15 Online:2014-03-15

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摘要： 目的 分析miR-30d在乳腺癌组织中的表达情况；体外研究miR-30d ASO对乳腺癌细胞侵袭和迁移的影响。方法 运用荧光定量PCR检测108例乳腺癌组织及对应正常组织中miR-30d的表达；通过miR-30dASO降低乳腺癌细胞中miR-30d的表达，采用transwell实验和划痕实验观察miR-30dASO对乳腺癌细胞侵袭和迁移能力的影响，同时运用western blot 的方法检测侵袭相关蛋白的表达变化。结果 在108例乳腺癌病例中，50.93%（55/108）的乳腺癌组织miR-30d表达明显高于对应正常组织（p<0.05）；与空白对照组和转染随机ASO组相比，miR-30d ASO可以显著降低miR-30d的表达（p<0.05）；Transwell实验和划痕实验结果显示转染miR-30dASO后，乳腺癌细胞的侵袭迁移能力明显下降，同时相关侵袭蛋白MMP2和MMP9表达下降（p<0.05）。结论 miR-30d在乳腺癌组织中表达上调, 降低miR-30d的 表达可有效抑制乳腺癌细胞的侵袭和迁移。miR-30d有可能成为乳腺癌侵袭转移调控的新靶点。

关键词: miR-30d, 乳腺癌, 侵袭, 迁移, 反义单核苷酸

Abstract: Objective To investigate the expression of miR-30d in breast cancer tissues and demonstrate the regulative effects of miR-30d ASO on the invasion and migration of breast cancer cells in vitro. Methods The expression of miR-30d in 108 breast cancer tissues and their adjacent tissues were detected by real-time quantitative PCR method. After transfection with miR-30dASO, the biological effects of miR-30d in breast cancer cells was measured by transwell assay and wound healing assay, and invasion-related protein expression was analyzed by western blot. Results The expression level of miR-30d was found to be overexpressed in 50.93%（55/108） of the breast cancer cases (p<0.05). miR-30d expression in breast cancer cells（transfection with miR-30d ASO）was significantly less than control group (p<0.05). Transwell and wound healing assay results showed that the invasion and migration ability decreased greatly after transfection with miR-30d ASO, furthermore,down-regulation of miR-30d resulted in obvious inactivation of MMP2 and MMP9. (p<0.05). Conclusion：miR-30d was overexpressed in human breast cancer. Reduce the expression of miR-30d can effectively inhibit the invasion and migration of breast cancer cells. miR-30d may become a new target for the regulation of invasion and migration in breast cancer.

Key words: miR-30d, breast cancer, Invasion, Migration, Antisense oligonucleotides