天津医药

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壳聚糖温敏凝胶对人牙龈成纤维细胞的生物学作用

彭伟,白宇宏,穆玉   

  1. 华北煤炭医学院口腔系
  • 收稿日期:2009-10-16 修回日期:2010-01-13 出版日期:2010-06-15 发布日期:2010-06-15
  • 通讯作者: 彭伟

Biological Effects of the Chitosan Thermosensitive Hydrogel on Human Gingival Fibroblasts Cells in Vitro.

  • Received:2009-10-16 Revised:2010-01-13 Published:2010-06-15 Online:2010-06-15
  • Contact: Peng Wei

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摘要： 目的: 了解壳聚糖温敏凝胶对人牙龈成纤维细胞(GFCs)的生物学作用, 为其局部应用治疗牙周病提供理论依据。方法: 采用组织块法分离培养人牙龈成纤维细胞，制备不同浓度的壳聚糖温敏凝胶浸提液，应用四甲基偶氮唑蓝(MTT)实验法检测其对GFCs的增殖状况的影响；用碱性磷酸酶(ALP)检测试剂盒检测细胞ALP活性以反映壳聚糖温敏凝胶对GFCs分化状况的影响。结果: 在培养初期24h，各浓度组浸提液与对照组比较、不同浓度组比较吸光度（A）值、ALP的OD值无显著性差异（P＞0.05）；培养48h后，各浓度组浸提液的吸光度（A）值、ALP的OD值均高于对照组，差异有统计学意义（P＜0.05），不同浓度的浸提液各组之间比较：25倍组与0.2倍组、5倍组与1倍组无显著性差异（P＞0.05），25倍组的吸光度（A）值、ALP的OD值均低于5倍组，差异有显著性（P＜0.05）。结论: 壳聚糖温敏凝胶有促进GFCs的增殖作用,能增强GFCs ALP活性,用于牙周病局部治疗,具有进一步的研究价值。

关键词: 人牙龈成纤维细胞, 牙周病, 温敏凝胶, 壳聚糖

Abstract: Objective::To evaluate the the biological effects of the chitosan thermosensitive hydrogel on human gingival fibroblasts (HGFs) and provide information about local application of the chitosan thermosensitive hydrogel on periodontal diseases. Methods：Human gingival fibroblasts were cultured in medium containing the chitosan thermosensitive hydrogel at different concentrations，MTT colorimetric assay were used to evaluate the proliferation and ALP kit was used to evaluate the ALP activity of the GFCs. Results：On the early cultures ，there was no significant difference between the 4 groups and the negative control group and each different concentrations of the chitosan thermosensitive hydrogel about the promotion on GFCs growth and proliferation（P＞0.05）. After 48h, the promotion of each different concentrations was better than those of control group,through the statistic analysis,there is statistical difference between the 4 groups and the negative control group（P<0.05）,there is no statistical difference between the the 25 times group and the 0.2 times group, 5 times group and the 1 times group （P＞0.05）.There is statistical difference between the the 25 times group and the 5 times group after 48h（P<0.05）. Conclusion：The results suggest that the chitosan thermosensitive hydrogel could promote the proliferation of the GFCs, local application of the chitosan thermosensitive hydrogel for treating periodontal diseases is feasible.

Key words: Human gingival fibroblast, periodontal disease, thermosensitive hydrogel, chitosan