天津医药

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血清嗜酸性粒细胞阳离子蛋白在5岁以下喘息儿童评估中的意义

杨金玲1,王秀芳2   

  1. 1. 济源市妇幼保健院
    2. 郑州大学第三附属医院
  • 收稿日期:2010-03-30 修回日期:2010-10-09 出版日期:2011-02-15 发布日期:2011-02-15
  • 通讯作者: 杨金玲

Expression and Significance of Serum ECP in Wheezing Children Under 5 Years of age

  • Received:2010-03-30 Revised:2010-10-09 Published:2011-02-15 Online:2011-02-15

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摘要： 摘要:目的 通过对5岁以下喘息儿童血清嗜酸细胞阳离子蛋白（ECP）水平的测定，并结合外周血嗜酸性粒细胞(EOS)计数，探讨其在5岁以下儿童喘息评估的临床意义。方法 随机选取于本院就诊的5岁以下反复喘息患儿53例，男43例，女10例，分为有特应质高危因素的喘息Ⅰ组（n=27）、无特应质高危因素的喘息Ⅱ组（n=26）。随机选取同期同年龄组本院外科疝气、肾结石等非感染性疾病术前患儿中随机抽取20例作为健康对照组(n=20) ，男11例，女9例。3组儿童均在治疗前抽取静脉血3ml,离心后留取血清标本，采用PharmaciaUniCAP系统检测ECP水平，同时采用伊红染色法进行外周血EOS计数。结果喘息Ⅰ组血清ECP水平和外周血EOS计数均明显高于喘息Ⅱ和对照组（P均＜0.05），而喘息Ⅱ组和对照组之间无统计学差异(P＞0.05); 相关分析显示血清中ECP与EOS呈正相关（r=0.786，P＜0.01）。结论 5岁以下反复喘息患儿血清ECP水平和外周血EOS计数与特应质高危因素关系密切，检测血清ECP水平，结合外周血EOS计数可作为识别5岁以下儿童喘息不同临床表型的实验室指标，有利于早期识别可能发展为持续喘息即哮喘的儿童，以指导儿童哮喘的早期干预 。

关键词: 儿童, 喘息, 嗜酸细胞阳离子蛋白, 嗜酸性粒细胞

Abstract: Abstract: Objective To explore the expression and clinical significance of serum eosinophil cationic protein (ECP) and peripheral blood eosinophile count(PBEC) in wheezing children under 5 years old. Methods Children with recurrent wheezing under 5 years old had been randomly enrolled including 43 boys and 10 girls. They were divided into 2 groups : wheezing group Ⅰ with atopic high risks （n=27）、wheezing group Ⅱ without atopic high risks （n=26）.Children without infectious diseases such as hernia and renal calculus had been enrolled randomly as normal control group（n=20）inculding 11boys and 9girls.After taking 3ml venous blood and centrifuging, the level ECPof in the serum was measured with PharmaciaUniCAP system, PBEC measurement was performed in the meantime by means of eosine staining . Results The serum levels of ECPand PBEC in wheezing groupⅠ were significantly higher than those of wheezing group Ⅱand the control group, the difference was statistically significant (P <0.05, P <0.01), while wheezing group Ⅱ group and the control group had no significant difference (P > 0.05); serum ECPand PBEC have positive correlation (r=0.786，P＜0.01). Conclusion The level serum of ECPand PBEC have an intimate relation with atopic high risks .The detections of them can be used as laboratory index to distinguish wheezing children with different clinical phenotype, early intervention and treatment should be given to the children who have a tendency to persistent wheezing.

Key words: children, wheezing, eosinophil cationic protein, eosinophile