天津医药

• 实验研究 •    

替米沙坦对胰岛素抵抗大鼠冠状动脉Rho/ROCK信号通路影响?

丁晓松,李卫萍,沈絮华,武星,李虹伟   

  1. 首都医科大学附属北京友谊医院
  • 收稿日期:2011-12-14 修回日期:2012-06-26 出版日期:2012-12-15 发布日期:2012-12-15
  • 通讯作者: 李虹伟

The Effect of Telmisartan on Rho / ROCK Signal Pathway in Insulin Resistance Rat’s Coronary Artery

  • Received:2011-12-14 Revised:2012-06-26 Published:2012-12-15 Online:2012-12-15

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摘要： 目的：观察胰岛素抵抗大鼠和正常大鼠冠状动脉RhoA及ROCK表达水平差异以及替米沙坦对胰岛素抵抗大鼠冠状动脉组织RhoA及ROCK表达水平的影响。方法：30只雄性SD 大鼠随机分为3组，正常对照组予普通饮食；胰岛素抵抗大鼠以高果糖饮食+链脲佐菌素（25mg/Kg）法造模，替米沙坦组在胰岛素抵抗基础上给予替米沙坦（5mg/kg/d）干预。干预4周后分离冠状动脉采用RT-PCR和 Western Blot法测定各组大鼠冠状动脉组织中RhoA、ROCK1、ROCK2的mRNA 和蛋白表达水平。结果：胰岛素抵抗组大鼠与正常对照组比较RhoA蛋白表达降低有统计学意义（P=0.018），ROCK1的mRNA的表达升高（P=0.042）。替米沙坦组大鼠RhoA蛋白表达水平较胰岛素抵抗组明显升高（P=0.043），具有统计学意义，与正常组比较无统计学差异（P=0.529）。其余组间RhoA 的mRNA、ROCK1的mRNA、ROCK1 、ROCK2蛋白表达均未达到统计学意义。结论：替米沙坦可部分逆转胰岛素抵抗大鼠冠状动脉RhoA蛋白水平降低，但对RhoA mRNA 、ROCK1和ROCK2mRNA及蛋白的表达水平的调节作用有限。

关键词: 胰岛素抵抗, 冠状动脉, 替米沙坦, RhoA, ROCK

Abstract: Objective:To observe the express level change of RhoA and ROCK signal pathway in insulin resistence Rat and To identify the role of telmisartan on the expression of RhoA and ROCK signal pathway in insulin resistence Rat small cornary artery tissue. Methods:There were 30 male Sprague-Dawley (SD) Rats was randomly divided into 3 groups,10 in each group.The control group was feed with normal diet ,10 in Insulin resistance group (creat model by high fructose diet plus Streptozotocin（25mg/Kg） injection ),10 in telmisatan group (insulin resistance plus telmisartan5mg/kg/d). 8 weeks later,Coronary arteries were isolated for the RT-PCR and Westen Blot assay to identify RhoA,ROCK1,ROCK2 mRNA and proten expression level.Results : Compared with control group, RhoA proten express level down regulated but ROCK1mRNA express level was up regulated in IR group. After interfere by telmisartan , RhoA proten expression was remarkably higher than IR group (P=0.043) .there were no statistical significance of RhoA,ROCK1mRNA and ROCK1,ROCK2 proten expression between Tel and IR group .Conclusions:Telmisartan can reverse the RhoA protein down regulation in IR rat’s coronary artery ,but only have a limited affect on RhoA mRNA ,ROCK1 and ROCK2mRNA and protein expression level in insulin resistance rat’s coronary artery.

Key words: Insulin Resistance, Coronary Arterys, Telmisartan, RhoA, ROCK