天津医药

• 实验研究 •    

代文、阿托伐他汀和匹格列酮对高糖培养的大鼠肾脏系膜细胞肾上腺髓质素和补体因子H的表达的影响

钟继娟   

  1. 南京医科大学第二附属医院
  • 收稿日期:2011-12-14 修回日期:2012-06-18 出版日期:2012-12-15 发布日期:2012-12-15
  • 通讯作者: 钟继娟

Effect of valsartan, atorvastatin and pioglitazone on the expression of adrenomedullin and complement factor H in mesangial cells cultured under high glucose condition

Ji-Juan ZHONG   

  • Received:2011-12-14 Revised:2012-06-18 Published:2012-12-15 Online:2012-12-15
  • Contact: Ji-Juan ZHONG

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摘要： 目的：观察代文、阿托伐他汀和匹格列酮对高糖培养的HBZY-1大鼠肾脏系膜细胞转化生长因子β1 （TGF-β1）、细胞外基质（ECM）以及肾上腺髓质素（AM）和补体因子H（Cfh）表达的影响，并探讨其作用机制。方法：实验分为低糖对照（LG）、高糖对照（HG）、高糖+代文（HD）、高糖+阿托伐他汀（HL）、高糖+匹格列酮（HP）5组，分别用酶联免疫吸附测定（ELISA）和放射免疫法（RIA）测定细胞上清TGF-β1、层粘连蛋白（LN）和Ⅳ型胶原的含量，用RT-PCR方法测定TGF-β1mRNA、CfhmRNA以及AMmRNA的表达情况。结果：高糖状态下培养的肾脏系膜细胞应用代文、阿托伐他汀和匹格列酮干预后细胞上清TGF-β1、LN和Ⅳ型胶原的含量较高糖对照组显著减低（P<0.01），TGF-β1mRNA，AMmRNA和CfhmRNA的表达亦较高糖对照组显著减低（P<0.01）。结论：代文、阿托伐他汀和匹格列酮具有一定的肾脏保护作用，其作用机制可能与其抑制TGF-β1的表达有关。药物干预后Cfh和AM的表达也减低，可能与TGF-β1的变化有关。

关键词: 糖尿病肾病, 肾小球系膜细胞, 缬沙坦, 吡咯类, 噻唑烷二酮类, 转化生长因子β1, 肾上腺髓质素, 补体因子H

Abstract: Objective: To observe the effect of valsartan, atorvastatin and pioglitazone on the expression of TGF-β1, extracellular matrix, adrenomedullin and complement factor H in mesangial cells cultured under high glucose levels and to investigate its mechanism. Methods: The experiment was divided into low glucose group(LG), high glucose group (HG), high glucose+valsartan(HD), high glucose+atorvastatin (HL)and high glucose+pioglitazone(HP) . ELISA and RIA were used to measure the content of TGF-β1，laminin and type Ⅳ collagenin of the cultured mesangial cells under high glucose conditions. RT-PCR was applied to determine the expression of TGF-β1mRNA, AMmRNA and CfhmRNA. Results: Valsartan, atorvastatin and pioglitazone could significantly decrease the concentrations of TGF-β1，laminin and type Ⅳ collagen in the supernatants for a certain extent compared with HG control group（P<0.01）,and they could also inhibit the expression of TGF-β1mRNA, AMmRNA and CfhmRNA of the cultured mesangial cells under high glucose conditions（P<0.01）. Conclusions: Valsartan, atorvastatin and pioglitazone showed a certain renoprotective functions which might relates to the inhibition of over-expression of TGF-β1 and extracellular matrix in mesangial cells induced by high glucose. Mean while, the expression of AM and Cfh was reduced, which might also relates to the variation of TGF-β1.

Key words: diabetic nephropathies, mesangial cells, valsartan, pyrroles, thiazolidinediones, transforming growth factor beta1, adrenomedullin, 补体因子H