天津医药

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血卟啉单甲醚光动力疗法对致龋变形链球菌及龋损表面形态的影响

李敏1,邹朝晖1,张蕾1,马莹莹1,阴慧娟2   

  1. 1. 天津医科大学口腔医院
    2. 天津医科大学生物医学工程系
  • 收稿日期:2010-05-20 修回日期:2010-10-31 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 邹朝晖

The Effect of HMME-mediated Photodynamic Therapy on Streptococcus mutans of Cariogenic Bacteria and Caries Morphology

  • Received:2010-05-20 Revised:2010-10-31 Published:2011-04-15 Online:2011-04-15

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摘要： 摘要 目的：探讨以血卟啉单甲醚(hematoporphyrin monomethyl ether，HMME)为光敏剂的光动力疗法（photodynamic therapy，PDT）在龋病预防中的作用。方法：采用变形链球菌接种Wistar大鼠磨牙，给予致龋饲料，建立龋病模型。将大鼠分为5组，分别用10 ng/L HMME-PDT（A组）、20 ng/L HMME-PDT（B组）、40 ng/L HMME-PDT（C组）、0.2 g/L氟化钠水溶液（D组）和0.9％的生理盐水(E组)处理各组大鼠磨牙，每周1次，连续4周，记录大鼠口腔变形链球菌生长情况。处死大鼠后用扫描电镜观察各组磨牙龋损区的表面形态。结果：第一周和第二周各组菌落数差异均无统计学意义（P>0.05），第三周B组、C组菌落数低于E组（P<0.05）。第四周A组、B组和C组菌落数均低于E组（P <0.05或P <0.01），C组菌落数低于A组和B组（P <0.05），扫描电镜观察发现C组与D组龋损破坏程度最轻且表面相对平滑。结论：PDT具有抑制龋病的作用，将为龋病的预防提供一种新的有效途径。

关键词: 血卟啉单甲醚, 光动力疗法, 变形链球菌, 龋损表面形态

Abstract: Abstract Objective: To study the role of photodynamic therapy using hematoporphyrin monomethyl ether as photosensitizer in the prevention of dental caries by establishing the caries rat model. Methods: Wistar rats, whose molars were inoculated with Streptococcus mutans (S. mutans), were fed with caries - inducing diet 2000# in order to establish the caries rat model. These rats were divided into five groups, whose molars were treated respectively with 10 ng/L HMME-PDT (group A), 20 ng/L HMME-PDT (group B), 40 ng/L HMME-PDT (group C), 0.2 g/L sodium fluoride as positive controls (group D), and with 0.9％sterile physiological saline as negative controls (group E) once a week for four weeks and recorded the changes of S. mutans every week. The rats were then killed. The surface morphology of dental caries of the rats was observed by scanning electron microscope. Results: The colony count in each group shows no significant difference in the first and second weeks ( P<0.05). The colony counts of group B and C are significantly reduced compared with group E in the third week ( P<0.05). In the fourth week the colony counts of group A、B and C are significantly reduced compared with group E (P<0.05 or P<0.01 ). Meanwhile the colony count of group C is significantly lower than both group A and B (P<0.05). The tooth enamel of both group C and D are found to be damaged most slightly, and the tooth surfaces of the two groups are relatively smooth. Conclusion: PDT has anticaries effect in vivo. PDT is thought to be a promising method in the prevention of caries.

Key words: HMME, PDT, streptococcus mutans, caries morphology