天津医药

• 实验研究 •    

清醒大鼠重度失血性休克模型指标建立及有效性评价

张艳1,王宇歆2,马涛3,姚丽静3,张响3,杨成民4,金学隆1   

  1. 1. 天津医科大学
    2. 天津协和生物科技发展有限公司；天津市南开医院
    3. 天津协和生物科技发展有限公司
    4. 天津协和生物科技发展有限公司；中国医学科院输血研究所
  • 收稿日期:2011-07-06 修回日期:2011-08-29 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 张艳

Establish and Effective evaluation of different Indexes of severe hemorrhagic shock model on Unrestrained Conscious Rats

  • Received:2011-07-06 Revised:2011-08-29 Published:2012-03-15 Online:2012-03-15

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摘要： 摘要: 目的 复制清醒大鼠重度失血性休克模型，建立有效的检测指标，评价模型的有效性，探讨重度失血性休克的病理生理机制。方法 将35只SD大鼠在清醒状态下经股动脉放其全身总血量的65%，造成重度失血性休克模型，并根据输入的液体不同随机分为三组，NaCl组（N=12）、全血组（N=12）和万汶组（N=13）。分别于放血前、放血末及输液后测定大鼠的动脉血压(Bp)、呼吸频率(R)、体温(T)及动脉血气和血乳酸盐(LD)。记录输液后24h及48h存活率。结果 与放血前比较，各组大鼠放血末Bp、T、PH、PCO2、HCT及BE明显降低（P<0.01）,PO2、血乳酸盐水平及SO2明显升高（P<0.01;P<0.05）。复苏2h后，与NaCl组相比，全血和万汶能明显地改善大鼠的Bp、PH、PCO2及BE，降低动脉血乳酸，改善代谢性酸中毒。结论（1）血气分析、血乳酸水平及存活率能作为评价失血性休克严重程度及预后的检测指标，有效性较好。(2)清醒大鼠重度失血性休克模型具有满意的重现性和有效性,适于不同复苏液抗休克性能的评价。

关键词: 失血性休克, 血气, 血乳酸, 碱剩余（BE）

Abstract: Abstract Objective: Establish severe hemorrhagic shock model On Unrestrained Conscious Rats, set up indexes and evaluate the validity of the indexes and the model to explore the severe hemorrhagic shock pathophysiology. Method: Thirty-Five Male Sprague-Dawley rats were hemorrhaged 65% of total blood volume via left Femoral artery on unrestrained consciousness, resulting in severe hemorrhagic shock model. They were randomly divided into three groups by different resuscitation fluids: NaCl (n=12), WB (whole blood) (n=12) and 6% HES (n=13). Measure the arterial pressure (Bp), respiratory rate (RR), Temperature (T), Arterial Blood Gas (ABG) and Lactic Acid (LD) at baseline, shock and 2 hours after resuscitation. Record f 24 and 48h survival after resuscitation. Results: Compared with the baseline, Bp,T,PH,PCO2,HCT and BE level of all rats decreased significantly(P<0.01); PO2, LD and SO2 level all increased significantly（P<0.01;P<0.05）. 2h after resuscitation, compared with NaCl, WB and 6%HES can improve the Bp, PH, PaCO2 and BE, decrease the LD level and improve the metabolic acidosis. Conclusion: (1) ABG, LD and survival can be as effective indexes of evaluating the severity and prognosis of severe hemorrhagic shock. (2) Severe hemorrhagic shock model On Unrestrained Conscious Rats has satisfactory reproducibility and effectiveness for the evaluation of anti-shock performance.

Key words: hemorrhagic shock, Arterial Blood Gas (ABG), Lactic Acid (LD), Base Excess (BE)