天津医药

• 实验研究 •    

慢性饮酒对于大鼠肺组织TGFβ-1和bFGF mRNA表达的影响

武俊平1,吴琦1,杜钟珍2,王星1   

  1. 1. 天津市海河医院
    2. 天津海河医院
  • 收稿日期:2011-05-03 修回日期:2011-08-13 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 武俊平

The effect of chronic alcohol ingestion on TGF-β1 and bFGF mRNA of lung tissues in rats

  • Received:2011-05-03 Revised:2011-08-13 Published:2012-03-15 Online:2012-03-15
  • Contact: Jun-Ping WU

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摘要： 目的：观察慢性饮酒后大鼠肺组织中肺组织转化生长因子-β1（TGF-β1）和碱性成纤维细胞生长因子（bFGF）mRNA的表达，探讨慢性饮酒对肺间质的影响。方法：健康雄性Sprague-Dawley大鼠20只随机分为无酒精液体饲料的对照组和酒精液体饲料的酒精组，每组10只。喂养16周后观察所有大鼠肺泡内炎性细胞浸润程度并评分，Masson染色观察肺间质胶原沉积情况，电镜观察肺组织超微结构变化，实时荧光定量PCR检测肺组织TGF-β1和bFGF mRNA的表达量。结果：光镜下肺泡内炎性细胞浸润程度评分酒精组高于正常对照组，差异有统计学意义（Z=50，p<0.05）。酒精组电镜可见线粒体膜和嵴均有不同程度融合、模糊不清，细胞间隔均可见不同程度的胶原纤维沉积。酒精组肺组织中TGF-β1和bFGF mRNA 表达量高于对照组，差异有统计学意义(t分别为3.702、3.487，均p<0.05)。结论：慢性饮酒可增加大鼠肺组织中TGF-β1、bFGF mRNA表达量，引起肺组织胶原沉积，引起肺泡炎症和肺间质疾病。

关键词: 肺疾病, 间质性, 乙醇, 转化生长因子β1, 成纤维细胞生长因子

Abstract: Objective To investigate the effect of chronic alcohol ingestion on lung interstitial tissue by detecting TGF-β1 and bFGF mRNA expression of lung tissue. Methods 20 male healthy Sprague-Dawley rats were randomly divided into non-ethanol(substitution of Maltose-Dextrin for EtOH) group(n=10) and ethanol(36% total calories) group(n=10). Lung tissues were extracted of all rats at 16 weeks after Lieber-DeCarli liquid diet. Histological change of lungs was evaluated by HE stain and transmission electron microscope(TEM).The mRNAs of transforming growth factor beta 1(TGF-β1) and basic fibroblast growth factor(bFGF) were detected by real time fluorescent quantitation polymerase chain reaction. Results In light microscope, the pulmonary inflammatory cell infiltrate score of alcohol group was significantly higher than that of the control group(Z=50， P<0.05). In TEM, mitochondrial membrane fused and fuzzy structure and collagen fiber deposition in cell compartment in different level were observed in alcohol group. The mRNA expressions of TGF-β1 and bFGF (34.74.±12.29,218.08±100.37) of alcohol group were both significantly increased compared with those of the control group(18.33±4.27,90.52±43.38,t=3.702,3.487,all p<0.05) Conclusion These findings suggested that chronic alcohol intake not only can cause alveolitis but also can cause lung interstitium disease by increasing the mRNA expressions of TGF-β1 and bFGF.

Key words: lung diseases, interstitial ethanol transforming growth factor beta1fibroblast growth factors rats, Sprague-Dawley