天津医药

• 实验研究 •    下一篇

复方儿茶止泻霜对腹泻大鼠结肠AQP3表达的影响

康乐1,高明2,丁宁3,宫婷2   

  1. 1. 大连大学医学院病理与病理生理学教研室
    2. 大连大学医学院中药学教研室
    3. 大连大学医学院生物化学教研室
  • 收稿日期:2013-06-08 修回日期:2013-07-23 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 康乐

Effects of Compound Catechu Anti-Diarrhea Ointment on Expression of Aquaporin3inColonic Tissues of Diarrhea Model Rats

KANG Le 1,GAO Ming 2,DING Ning 3,GONG Ting 2   

  1. 1. Department of Pathophysiology,Medical College of Dalian University
    2. Department of Traditional Chinese Medicine,Medical College of Dalian University
    3. Department of Biochemistry,Medical College of Dalian University
  • Received:2013-06-08 Revised:2013-07-23 Published:2013-11-15 Online:2013-11-15
  • Contact: KANG Le

PDF (PC)

2174

摘要：

【摘要】 目的 观察复方儿茶止泻霜对腹泻大鼠模型结肠组织水通道蛋白3（AQP3）表达的影响，初步探讨其治疗腹泻的机制。 方法  将40只SD大鼠随机分为空白对照组、模型对照组、阳性对照组和复方儿茶止泻霜组4组，各10只。模型对照组涂6 g空白基质霜剂，阳性对照组灌胃2 mL小檗碱悬浊液，复方儿茶止泻霜组涂6 g复方儿茶止泻霜，2次/d，给药7 d。采用番泻叶灌胃的方法建立大鼠腹泻模型，对除空白对照组以外的3组大鼠按20 mL/kg灌服20%番泻叶提取液。用智能水分测定仪测定粪便含水率，免疫组化法检测各组结肠组织AQP3的表达。  结果    模型对照组粪便含水率（64.09±0.41）%明显高于其他3组（F=53.879，P＜0.05）；复方儿茶止泻霜组（48.83±1.08）%与阳性对照组（46.87±2.19）%间差异无统计学意义。AQP3阳性细胞主要表达于结肠黏膜层，模型对照组染色指数（0.85±0.18）低于其他3组（F=14.971，P＜0.05）；复方儿茶止泻霜组（1.30±0.18）与阳性对照组（1.37±0.14）间差异无统计学意义。 结论   复方儿茶止泻霜可显著减少腹泻大鼠粪便含水率，其机制可能与上调结肠组织AQP3的表达有关。 

关键词: 水通道蛋白3, 复方儿茶止泻霜, 腹泻, 结肠, 水代谢

Abstract:

[Abstract]   Objective  To investigate the effect of compound catechu anti- diarrhea ointment on the expression of
aquaporin3(AQP3) in the colonic tissues of diarrhea rat model, and the mechanism thereof.   Methods   Forty SD rats were randomly divided into four groups: blank control group, model control group, positive control group and compound catechu anti-diarrhea ointment group. Rats were given6g blank matrix cream in model control group,2mL suspension of berberine in positive control group and compound catechu anti-diarrhea ointment in compound catechu anti-diarrhea ointment group, two times/d for 7d. The rat model of diarrhea was established by using senna intragastric administration. The water content of feces was measured. The expression of AQP3in colonic tissues was detected by immunohistochemistry assay.   Results   The water contents of feces were significantly higher in model control group (64.09±0.41) %than those of other three groups(F=53.879，P＜0.05). There was no significant difference in the water content of feces between compound catechu anti-diarrhea ointment group (48.83±1.08)% and positive control group (46.87±2.19)%. The AQP3-positive cells were mainly expressed in the intestinal mucosa. The dyeing index was significantly lower in model control group (0.85±0.18) than that of other three groups (F=14.971，P＜0.05). There was no significant difference in the dyeing index between compound catechu anti-diarrhea ointment group (1.30±0.18) and positive control group (1.37±0.14).    Conclusion   Compound catechu anti-diarrhea ointment can significantly reduce the water contents of feces, which may be related to the increased AQP3expression in colonic tissues.

Key words: aquaporin 3, compound catechu anti- diarrhea ointment, diarrhea, colon, water metabolism