天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (4): 400-403.doi: 10.11958/j.issn.0253-9896.2015.04.018

• 专题研究·细菌与耐药 • 上一篇    下一篇

志贺菌1、2 类整合子及ISCR1 携带情况与耐药性的关系

董利娟1，杨贤1，王俊1，祁伟1△，吕星2，梁帆3   

  1. 1天津医科大学第二医院感染研究所(邮编300211)；2天津医科大学总医院；3天津市儿童医院
  • 收稿日期:2014-06-18 修回日期:2014-11-14 出版日期:2015-04-15 发布日期:2015-04-13
  • 通讯作者: 祁伟 E-mail:qiweiwyx@yahoo.com
  • 作者简介:董丽娟（1989），女，硕士在读，主要从事志贺菌耐药性方面研究

Carriage rates of class 1, class 2 integrons as well as ISCR1 in Shigella isolates and their relationship with drug resistance

DONG Lijuan1, YANG Xian1, WANG Jun1, QI Wei1△, LYU Xing2, LIANG Fan3   

  1. 1 Second Hospital of Tianjin Medical University Institute of Infectious Disease, Tianjin 300211, China; 2 Tianjin Medical University General Hospital; 3 Tianjin Children's Hospital
  • Received:2014-06-18 Revised:2014-11-14 Published:2015-04-15 Online:2015-04-13
  • Contact: QI Wei E-mail:qiweiwyx@yahoo.com

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摘要： 摘要：目的了解天津地区志贺菌1 类、2 类整合子及插入序列共同区(ISCR1)携带情况及其与耐药性的关系。方法K-B 纸片扩散法测定临床分离的159 株志贺菌的药敏情况。以煮沸法制备细菌总DNA 作为PCR 扩增模板。采用PCR 方法扩增1 类、2 类整合子及ISCR1 并测序分析。PCR 产物直接测序，结果经BLAST 程序与Gen⁃ Bank 数据库标准株比对分析。结果159 株志贺菌中，53 株福氏志贺菌对四环素的耐药率最高88.68%，其次是链霉素81.13%、氯霉素及SMZco 均为56.60%，多重耐药率77.36%；106 株宋氏志贺菌对氨苄西林的耐药率最高 97.17%，其次是SMZco95.28%、四环素83.96%、庆大霉素76.42%，多重耐药率98.11%。1 类整合子阳性118 株，其中典型1 类整合子23 株，共有5 种基因盒，分别为aadA2、aadA1、dfrⅠ、blaoxa-10 及blaoxa-1；非典型1 类整合子95 株，基因包括intI1、aadA、blaoxa-1 和IS1 等；2 类整合子阳性89 株，其基因盒有dfrA1、satl 及aadA1；1 类、2 类整合子同时阳性的菌株70 株。未发现ISCR1 阳性菌株。整合子阳性菌株中多重耐药率高于整合子阴性菌株(90.65% vs 50%， P < 0.05)。结论1、2 类整合子广泛存在于志贺菌中且与志贺菌的多重耐药相关。

关键词: 志贺菌属；整合子类；耐药性, 微生物；序列共同区

Abstract: Abstract: Objective To explore carrying rates of class 1, class 2 integrons as well as ISCR1 in Shigella isolates and their connection with drug resistance. Methods Antibiotic sensitivities were detected by K-B disk diffusion in 159 clinical isolates. Total bacteria DNA was prepared through boiling the isolates and the DNA was then used as template for PCR am⁃ plification. PCR, ZSCR1 and sequencing analyse of integrons were applied to all of them. Results were compared by Blast and GenBank. Results Antibiotic sensitivity results showed that in the S. flexneri strains the incidence of resistance to tet⁃ racycline and streptomycin were 88.68% and 81.13% in the S. flexneri strains while the incidence of resistant to chloram⁃ phenicol and trimethoprim-sulfamethoxazol were both 56.60%, and the incidence of multidrug drug resistance was 77.36%. In the sonnei strains, the incidence of resistance to ampicillin, trimethoprim-sulfamethoxazo were 97.17% and 95.28%, 83.96% and 76.42% respectively, and the incidence of multidrug resistance was 98.11%. Among all isolates, 118 were class 1 integron positive , 70 were class 2 integron positive and 89 were double positives. For those 118 isolates that are positive of class 1 integron, 23 were typical while 95 weres atypical. The gene cassettes of typical class 1 integrons contains aadA2, aa⁃ dA1, dfrⅠ, blaoxa-10 and blaoxa-1. IntI1, aadA, blaoxa-1 and IS1 were included in the gene cassetes of the atypical class 1 integrons. Class 2 integrons positive isolates carried gene cassttes which include dfrA1, satl and aadA1. No ISCR1 was found in any isolate. Integron carriage strains were closely associated with higher rate of multiple antibiobic resistance com⁃ pared with the organisms without integrons (90.65%,50%, P < 0.05). Conlusion Class 1 and class 2 integrons were widely existence in Shigella isolates and were related to the multidrug resistance.

Key words: shigella, integrons, drug resistance, microbiol, sequence common region