天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (6): 642-645.doi: 10.11958/j.issn.0253-9896.2015.06.017

• 临床研究 • 上一篇    下一篇

IGF2BP3低甲基化与结肠癌组织分化的关系

常江 1， 王颖 2， 柳利 1， 王满仓 1   

  1. 1内蒙古自治区巴彦淖尔市医院普外科 （邮编015000）， 2肾内科
  • 收稿日期:2014-10-16 修回日期:2015-01-12 出版日期:2015-06-15 发布日期:2015-06-10
  • 通讯作者: 常江 E-mail:494367392@qq.com

The relationship between IGF2BP3 hypomethylation with colon tumor differentiation

CHANG Jiang1, WANG Ying2, LIU Li1, WANG Mancang1   

  1. 1 Department of General Surgeon, 2 Department of Nephrology, Inner Mongolia Bayannaoer City Hospital,
    Inner Mongolia 015000, China

  • Received:2014-10-16 Revised:2015-01-12 Published:2015-06-15 Online:2015-06-10

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摘要： 目的 探讨 IGF2BP3 低甲基化与结肠癌组织分化之间的关系。方法 收集 2011 年 3 月—8 月在我院就诊的结肠癌组织标本 41 例， 其中高分化腺癌 19 例， 中分化腺癌 12 例， 低分化腺癌 10 例， 同时收集结肠炎标本 12例作为对照。免疫组化和 Western blot 检测标本 IGF2BP3 表达。改进 ELISA 法检测标本 DNA 总甲基化水平， 甲基化特异性 PCR（MS-PCR）检测 IGF2BP3 甲基化水平。结果 免疫组化和 Western blot 结果示结肠癌组织 IGF2BP3表达高于结肠炎组织 （P < 0.05）， 低分化结肠癌组 IGF2BP3 表达水平最高， 中、 高分化程度间表达无明显差异。结肠癌组基因总甲基化水平和 IGF2BP3 甲基化水低于结肠炎组（均 P < 0.05）， 且随着结肠组织分化程度降低， IGF2BP3甲基化水平依次降低（P < 0.05）。结论 IGF2BP3 甲基化水平与结肠癌分化程度密切相关， 在调控结肠癌组织IGF2BP3 表达中具有重要价值。

关键词: 胰岛素样生长因子 2 信使 RNA 结合蛋白 3, 结肠癌, DNA 甲基化, 分化, 免疫组织化学, 甲基化特异性 PCR

Abstract: Objective To investigate the relationship between IGF2BP3 hypomethylation with colon tumor differentiation. Methods Tissue samples from 41 colorectal cancer were collected from March 2011 to August 2011 in our hospital,among which 19 cases were well-differentiated adenocarcinoma, 12 cases were of moderately differentiated adenocarcinoma and 10 cases were of poorly differentiated adenocarcinoma. Meanwhile biopsy samples from 12 cases of colonic colitis were collected as a control. The expression of IGF2BP3 was assessed by immunohistochemistry and Western blot. An innovate of ELISA technique was used to examine global methylation levels while IGF2BP3 methylation level was tested by methylation specific PCR technique. Results The expressions of IGFBP3 are higher in all colon cancer groups than that in colitis (P <0.05). The expression of IGFBP3 in poorly differentiated adenocarcinoma is higher than that in all the other groups, but there is no significant difference between its expressions in the well differentiated group and in the moderately differentiated adenocarcinoma group. The global DNA level and IGFBP3 methylation level of every colon cancer group is lower than those in colitis (P < 0.05). Also, a tendency of decreasing global DNA and IGFBP3 methylation status was observed comparing well differentiated towards poorly differentiated carcinomas (P < 0.05). Conclusion IGF2BP3 expression in colorectal cancer is associated with differentiation of colon cancer tissue. A lower global and IGF2BP3 DNA methylation suggest a worse differentiation of colon cancer. DNA hypomethylation may therefore play a regulatory role in the expression of IGF2BP3 in colon cancer tissue.

Key words: IGF2BP3, colonic neoplasms, DNA methylation, differentiation, immunohistochemistry, methylation-specific
PCR