天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 865-868.doi: 10.11958/20150340

• 实验研究 • 上一篇    下一篇

虾青素对人肺癌 A549 细胞裸鼠移植瘤放疗敏感性的影响

吴春涛,苑威,刘铁楠,张晋冀,李长仔,胡宝山   

  1. 1 华北理工大学附属医院肿瘤外科（邮编 063000）， 2药学部， 3 心内科
  • 收稿日期:2015-11-20 修回日期:2016-03-25 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 吴春涛 E-mail:568060@qq.com
  • 作者简介:吴春涛（1976）， 男， 副主任医师， 医学学士， 主要从事肿瘤治疗学方面的研究
  • 基金资助:
    中国煤炭工业协会指导性课题

Astaxanthin enhanced radiotherapy sensitivity of human lung cancer A549 cells transplanted in nude mice

WU Chuntao1, YUAN Wei2, LIU Tienan3, ZHANG Jinji1, LI Changzai1, HU Baoshan   

  1. 1 Department of Oncological Surgery, 2 Department of Pharmaceutical, 3 Department of Cardiology, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, China
  • Received:2015-11-20 Revised:2016-03-25 Published:2016-07-15 Online:2016-07-15
  • Contact: WU Chuntao E-mail:568060@qq.com

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摘要： 目的 探讨虾青素对人肺癌 A549 细胞裸鼠移植瘤放射治疗敏感性的影响。 方法 20 只 BALB/c Nude 裸鼠随机均分为 4 组： 对照组（小鼠灌胃 10%DMSO 的超纯水）， 虾青素组（小鼠灌胃 10%DMSO 的虾青素悬液， 剂量为 50 mg/kg， 每隔 1 d 给药 1 次， 共给药 7 次）， 照射组（小鼠灌胃 10%DMSO 的超纯水， 肿瘤生长部位使用加速器局部照射， 5 Gy/次， 总剂量 15 Gy）及联合组（同时进行虾青素灌胃和局部照射处理）。 肿瘤最短直径达到 5 mm 以上开始实验， 每隔 1 d 测量移植瘤长短直径计算肿瘤体积并绘制移植瘤生长曲线， 末次给药后第 2 天处死小鼠， 取肿瘤块称质量并将其制成石蜡切片。 免疫组化方法检测石蜡切片内肿瘤细胞增殖抗原 Ki-67、磷酸化 STAT3（p-STAT3）表达及细胞凋亡（Tunnel 染色）情况。 结果 与对照组比较， 虾青素组、照射组及联合组瘤体生长速度减慢（P < 0.05）， 以联合组瘤体生长速度最慢。 对照组、虾青素组、照射组和联合组瘤体质量、Ki-67、p-STAT3 表达水平均依次降低， 抑瘤率、细胞凋亡率均依次升高， 组间多重比较差异均有统计学意义（P < 0.05）。 结论 虾青素能够通过抑制 p-STAT3 表达增加肺癌 A549 细胞裸鼠移植瘤的放疗敏感性。

关键词: 肺肿瘤, 癌, 辐射耐受性, 放射疗法, 小鼠, 裸, 虾青素, 肺癌 A549 细胞

Abstract: Objective To observe the effect of astaxanthin on radiotherapy sensitivity of lung cancer A549 cells transplanted in nude mice. Methods Twenty BALB/c nude mice were divided into four groups: control group (mice were gavaged with pure water containing with 10% DMSO), astaxanthin group (mice were gavaged with astaxanthin suspension containing with 10% DMSO, astaxanthin was given to mice with the dose of 50 mg/kg on the first day, and every other day in the following days with a total of 7 times), radiotherapy group (mice were gavaged with pure water containing with 10% DMSO, the tumor site was given local radiotherapy with a dose of 5 Gy per time and the total dose was 15 Gy) and combination group (mice were given 50 mg/kg astaxanthin and radiotherapy with 15 Gy total irradiated dose). When the minor axis of the tumor reached 5 mm we began experiment. Tumor growth curve was measured by detecting the line of apsides every other day. Mice were killed on the second day after the last time of astaxanthin administration. Weights of tumor were measured by a balance and then tumor mass was processed into paraffin sections. Expressions of proliferating tumor cell antigen Ki-67, phosphorylated-signal transducers and activators of transcription (p-STAT3), and cell apoptosis (measured by terminal deoxynucleotidyl transferase mediated dUTP nick- end labeling, Tunnel) were detected by immunohistochemistry. Results Compared with control group, the transplanted tumor growth rate slowed down in other three groups (P < 0.05), and tumor growth was the most slowly in the combination group. Tumor weight, Ki-67 and p-STAT3 expressions were decreased gradually in turn in control group, astaxanthin group, radiotherapy group and combination group. The anti- tumor rate and percentage of cell apoptosis were increased gradually in turn. There was significant difference between groups by multiple comparison statistics(P < 0.05). Conclusion Astaxanthin enhances radiotherapy sensitivity of human lung cancer A549 cells in nude mice by down-regulating the expression of p-STAT3.

Key words: lung neoplasms, carcinoma, radiation tolerance, radiotherapy, mice, nude, Astaxanthin, lung cancer A549 cell