天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (12): 1436-1440.doi: 10.11958/20160705

• 实验研究 • 上一篇    下一篇

法舒地尔对蛛网膜下腔出血后大鼠脑皮质Bcl-2和Bax 蛋白表达的影响

缪春明， 沈维高， 王雷， 魏盾△   

  1. 北华大学附属医院神经外科 （邮编 132011）
  • 收稿日期:2016-07-19 修回日期:2016-09-18 出版日期:2016-12-15 发布日期:2017-01-26
  • 通讯作者: 魏盾 △通讯作者 E-mail：miaochunming218@163.com E-mail:j_03357@126.com
  • 作者简介: 缪春明 （1967）， 男， 主任医师， 博士， 硕士研究生导师， 主要从事脑外科基础临床研究
  • 基金资助:
    吉林省教育厅 “十二五” 科学技术研究 ［吉教科合字 （2012） 第 149 号］

Effects of fasudil on the expressions of Bcl-2 and Bax in cerebral cortex of rats with subarachnoid hemorrhage

MIAO Chunming, SHEN Weigao, WANG Lei, WEI Dun△   

  1. Department of Neurosurgery, the Affiliated Hospital of Beihua University, Jilin 132011, China
  • Received:2016-07-19 Revised:2016-09-18 Published:2016-12-15 Online:2017-01-26
  • Contact: WEI Dun △Corresponding Author E-mail： miaochunming218@163.com E-mail:j_03357@126.com

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摘要： 摘要： 目的 探讨法舒地尔对蛛网膜下腔出血 （SAH） 后大鼠脑皮质 B 淋巴细胞瘤-2 （Bcl-2） 和 Bax 蛋白表达的影响。方法 30 只大鼠随机均分为假手术组、 SAH 组及 SAH+法舒地尔组。采用枕大池二次注血法制备大鼠 SAH 模型， 假手术组将自体血替换为生理盐水， SAH+法舒地尔组为第 2 次注血后 30 min 腹腔注射法舒地尔注射液， 剂量为 3 mg/kg。24 h 后比较各组大鼠术后的一般情况和神经行为学评分， TUNEL 染色法观察脑皮质细胞凋亡情况， 免疫组化染色和 Western blotting 检测 Bcl-2 和 Bax 蛋白表达情况。结果 与假手术组相比， SAH 组和 SAH+法舒地尔组大鼠出现明显的神经运动功能障碍体征； SAH 组神经行为学评分 （2.68±0.31） 低于假手术组 （5.00±0.00）， SAH+法舒地尔组 （3.27±0.35） 高于 SAH 组 （P＜0.05）。SAH 组和 SAH+法舒地尔组均可见明显的神经细胞凋亡， 且 SAH+法舒地尔组凋亡程度轻于 SAH 组。 SAH 组的 Bcl-2 表达水平低于假手术组， 而 Bax 表达水平高于假手术组 （P＜ 0.05）； SAH+法舒地尔组 Bcl-2 表达水平较 SAH 组升高， 而 Bax 表达量较 SAH 组降低 （P＜0.05）。结论 法舒地尔可明显改善 SAH 引起的神经功能损伤， 其作用机制可能与调节凋亡相关蛋白 Bcl-2和 Bax 的表达有关。

关键词: 蛛网膜下腔出血, 基因, bcl-2, bcl-2 相关X 蛋白质, 细胞凋亡, 法舒地尔

Abstract: Abstract： Objective To investigate the effects of fasudil on expressions of Bcl-2 and Bax in cerebral cortex of model rats with subarachnoid hemorrhage (SAH). Methods Thirty rats were divided into sham operation group, SAH group and SAH+ fasudil group, 10 rats in each group. Double injection of blood into occipital cistern method was used for SAH model in SAH group and SAH+ fasudil group. In the sham operation group, the blood injection was instead by normal saline. In the SAH+ fasudil group, at 30 min after the second injection of blood, rats were administrated with intraperitoneal injection of fasudil at a dose of 3 mg/kg. The general situation, neurological score, TUNEL staining for cortex cell apoptosis, immune histochemical staining and Western blotting assay for Bcl- 2 and Bax protein expression were compared 24 h after the operation between the three groups. Results Compared with the sham operation group, rats in SAH group and SAH + fasudil group appeared obvious neurological deficits. The neurological score was significantly lower in SAH group (2.68± 0.31) than that of sham operation group (5.00±0.00). The neurological score was significantly higher in SAH + fasudil group (3.27±0.35) compared with that of SAH group (2.68±0.31, P＜0.05). There was obvious cell apoptosis in SAH group and SAH + fasudil group, and the apoptosis was less in SAH + fasudil group than that of SAH group (P＜0.05). The level of Bcl- 2 expression was significantly lower in SAH group than that of sham operation group, and Bax expression was significantly higher in SAH group than that of sham operation group (P＜0.05). The level of Bcl-2 expression was significantly higher in SAH + fasudil group than that of SAH group, but Bax expression was significantly lower in SAH + fasudil group than that of SAH group (P＜0.05). Conclusion Fasudil can improve the neurological damage in rats with SAH, which may be related with the regulation of apoptosis related proteins Bcl-2 and Bax.

Key words: subarachnoid hemorrhage, genes, bcl-2, bcl-2-associated X protein, apoptosis, fasudil