天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (1): 17-20.doi: 10.11958/20160808

• 实验研究 • 上一篇    下一篇

2 种不同方法构建肝纤维化小鼠 70%肝切除模型的比较 #br# #br#

张迪 1， 张雅敏 2△， 崔子林 2， 杨龙 2， 李阳 2   

  1. 1 天津医科大学一中心临床学院 （邮编 300192）； 2 天津市第一中心医院肝胆外科

  • 收稿日期:2016-08-10 修回日期:2016-10-29 出版日期:2017-01-15 发布日期:2017-01-15
  • 通讯作者: 张雅敏 E-mail:zhangyamin@medmail.com.cn
  • 基金资助:
    天津市应用基础与前沿技术研究计划;国家自然科学基金面上项目

Comparison of 70% hepatectomy model established by two different methods in liver fibrosis mice

ZHANG Di1， ZHANG Ya-min2△， CUI Zi-lin2， YANG Long2， LI Yang2 #br# #br#   

  1. 1 First Central Clinical College of Tianjin Medical University, Tianjin 300192, China; 2 Department of Hepatobiliary Surgery, Tianjin First Central Hospital
  • Received:2016-08-10 Revised:2016-10-29 Published:2017-01-15 Online:2017-01-15
  • Supported by:
    ;National Natural Science Foundation of China

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摘要： 摘要： 目的 探求一种稳定可靠的建立肝纤维化小鼠 70%肝切除模型的方法。方法 66 只 C57BL6 小鼠按随 机数字表法抽取 6 只作为对照组， 剩余 60 只小鼠先诱导肝纤维化后， 与对照组对比观察小鼠肝纤维化模型肝脏的 纤维化程度， 随机均分为传统组 （单纯结扎摘除肝叶， 30 只） 和改良组 （阻断血流后摘除肝叶， 30 只） 建立 70%肝切除 模型。每组分别于肝切除术前和术后 12 h、 24 h、 48 h、 72 h 5 个时间点处死 6 只小鼠， 收集肝脏组织及血液标本。记 录术后 2 组的成活率和并发症的发生情况； 检测血清丙氨酸转氨酶 （ALT）、 天冬氨酸转氨酶 （AST） 水平， 观察肝损伤 情况； 计算肝质量/体质量比值， 并检测增殖细胞核抗原 （PCNA） 表达来观察 2 组肝再生状态。结果 （1） 传统组和改 良组小鼠肝纤维化模型均构建成功， 可用于 70%肝切除。（2）肝部分切除术后， 相比传统组， 改良组小鼠的成活率显 著升高 （96.67% vs. 73.33%）， 术中出血、 术后腔静脉狭窄及胆漏并发症的发生率明显降低 （P＜0.05）。（3） 术后 12 h 和 24 h， 传统组的 ALT 和 AST 水平高于改良组， 而各组内 ALT、 AST 均于术后 12 h 时明显升高， 之后随时间呈降低趋 势（P＜0.05）。（4）术后 2 组肝质量/体质量比值于术后 12 h 明显降低， 之后随时间延长呈缓慢增长趋势， PCNA 阳性 细胞数均于术后开始升高， 在 48 h 达到高峰后下降（P＜0.05）， 但各时间点 2 组间差异均无统计学意义。结论 通 过阻断肝叶血流构建肝纤维化小鼠 70%肝切除模型， 可显著提高模型的成功率， 降低并发症的发生率。

关键词: 肝切除术, 肝再生, 手术后并发症, 肝硬化,实验性, 小鼠, 近交 C57BL, 疾病模型, 动物

Abstract: Abstract：Objective To explore a reliable method of 70% hepatectomy model in liver fibrosis mice. Methods Sixty-six C57BL6 mice were randomly devided into control group (n=6), the traditional group (n=30, ligation and removal liver lobe) and improved group (n=30, removal of liver lobe after blocking blood flow). Those 60 mice were induced liver fibrosis firstly, then randomly divided into six mice in each group, and were sacrificed at preoperative, 12, 24, 48 and 72 hours after liver resection. Liver tissues and blood samples were collected. The survival rate and incidence of complications were recorded and compared between two groups. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured to observe the liver injury after 70% hepatectomy. The ratio of liver weight to body weight and the expression of proliferating cell nuclear antigen (PCNA) were also measured to observe the difference of liver regeneration between the two groups. Results (1) Compared to the pathological control group, liver fibrosis model was established successfully in both traditional group and improved group, which can be used in 70% hepatectomy. So the followup experiment can be undertook timely. (2) Compared to traditional group, the survival rate was improved significantly in improved group (96.67% vs. 73.33%), and the incidence of complications was significantly lower (P＜0.05). (3) The ALT and AST levels were higher 12 h and 24 h after operation in traditional group than those of improved group (P＜0.05), while ALT and AST levels were increased first 12 h after operation and then decreased in both groups (P＜0.05). (4) The liver/body weight ratio showed a decreasing trend 12 h after hepatectomy in two groups. The expression of PCNA increased at the beginning of postoperative, and reached its peak at 48 h (P＜0.05). However, there was no significant difference at each time point between the two groups. Conclusion By blocking blood flow to establish 70% hepatectomy model in liver fibrosis mice, we can significantly improve the success rate of the model, and reduce the incidence of complications.

Key words: hepatectomy, liver regeneration, postoperative complications, liver cirrhosis, experimental, mice, inbred
C57BL, disease models, animal