天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (3): 261-264.doi: 10.11958/20150028

• 专题研究-骨疾病 • 上一篇    下一篇

富血小板纤维蛋白对牵引成骨区BMP-6表达的影响

付颖,董庆文,王稚英   

  1. 辽宁医学院附属第二医院口腔内科
  • 收稿日期:2015-07-13 修回日期:2015-09-15 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: 付颖 E-mail:lykqfy@126.com
  • 作者简介:付颖（1977）， 女， 副教授， 硕士， 主要从事口腔医学研究
  • 基金资助:
    辽宁省自然科学基金项目（2015020352）；辽宁省教育厅科学研究一般项目（L2013322）

The changes of expressions of BMP-6 with PRF on mandibular distraction osteogenesis

FU Ying, DONG Qingwen, WANG Zhiying   

  1. Department of Oral Medicine, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, China
  • Received:2015-07-13 Revised:2015-09-15 Published:2016-03-15 Online:2016-03-15
  • Contact: Ying FU E-mail:lykqfy@126.com

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摘要： 目的 探讨应用富血小板纤维蛋白（platelet-rich fibrin,PRF）对牵引成骨区骨形态发生蛋白-6（bone morphogenetic protein 6,BMP-6）表达的影响。方法 25只大耳白兔随机分5组，分别行双侧下颌骨皮质骨切开术，一侧下颌骨牵引间隙放置PRF膜，作为实验组，对侧作为对照组，分别于稳定期第1、3、7、14、28天处死一组动物，切取牵引间隙处骨痂行HE染色和BMP-6免疫组织化学染色，细胞图像分析仪测量牵引间隙处骨痂BMP-6表达情况。结果 下颌牵引延长后牵引间隙均有新骨形成，免疫组化染色BMP-6主要定位于成骨细胞的胞浆中。实验组在稳定期1、3、7天BMP-6表达的阳性细胞率和阳性面积百分比均高于对照组（P＜0.05），稳定期14、28天BMP-6表达的阳性细胞率和阳性面积百分比均与对照组比较差异无统计学意义。结论 PRF能促进兔下颌骨牵引成骨区新骨的生成，BMP-6可能在牵引成骨过程的早期调控组织细胞应力信号传递，发挥成骨作用。

关键词: 骨形态发生蛋白质类, 骨生成, 牵张, 兔, 富血小板纤维蛋白, 骨形态发生蛋白-6

Abstract: Objective To investigate the effects of PRF on mandibular distraction osteogenesis and the expression of BMP-6 in the period of distraction osteogenesis. Methods Twenty-five mature rabbits were randomly divided into five groups, Mandibular osteotomies were performed corticectomy in bilateral mature rabbits,PRF were implanted in the one side of mandibles as experimental group. Another side of madibular as control group,The rabbits were sacrificed and the distracted calluses were harvested and processed for HE and BMP-6 immunohistochemistry on the 1st,3th,7th,14st and 28th day after the end of distraction, respectively.The expressions of BMP-6 in the distracted calluses were analyzed by cell digital imaging software. Results The regenerated bone was found in the distraction gap after mandibular lengthening.BMP-6 were co-localized in cytoplasm of osteoblasts and newly embedded osteocytes.Compared with control group, the positive cell rate and positive area percentage of BMP-6 were higher on the 1st, 3th and 7th day after the distraction in experimental group(P＜0.05).Then there was no statistical significance on the positive cell rate and positive area percentage of BMP-6 on the 14st and 28th day after the distraction in experimental group.Conclusion? PRF could accelerate bone formation of mandibular distraction osteogenesis in rabbit,BMP-6 may play important roles at the early stage of mandibular distraction.

Key words: bone morphogenetic proteins, osteogenesis,distraction, rabbits, platelet-rich fibrin, bone morphogenetic protein 6