天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (8): 1005-1009.doi: 10.11958/20150213

• 临床研究 • 上一篇    下一篇

骨桥蛋白、 caspase-3 及突变型P53在胶质瘤中的表达研究

郭可盈， 曾苏婷， 范伟， 曹思源， 王莹， 陆锦标， 王东林△   

  1. 南通大学医学院病理学系 （邮编226001）
  • 收稿日期:2015-10-10 修回日期:2016-02-17 出版日期:2016-08-15 发布日期:2016-08-22
  • 通讯作者: 王东林 E-mail:donglin@ntu.edu.cn E-mail:664421769@qq.com
  • 作者简介: 郭可盈 （1994）， 女， 本科在读， 主要从事胶质瘤研究
  • 基金资助:
    国家自然科学基金资助项目 （81272789）； 南通大学大学生创新训练计划项目 （2014091）

The study on expressions of osteopontin， caspase-3 and mt-P53 in glioma

GUO Keying, ZENG Suting, FAN Wei, CAO Siyuan, WANG Ying, LU Jinbiao, WANG Donglin△   

  1. Department of Pathology， School of Medicine, Nantong University, Nantong 226001, China
  • Received:2015-10-10 Revised:2016-02-17 Published:2016-08-15 Online:2016-08-22
  • Contact: WANG Donglin E-mail:donglin@ntu.edu.cn E-mail:664421769@qq.com

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摘要： 摘要： 目的 研究胶质瘤中骨桥蛋白 （OPN）、 半胱氨酸天冬氨酸蛋白酶-3 （caspase-3） 和突变型 P53 （mt-P53） 蛋白的表达意义和相关性。方法 选取脑胶质瘤 （胶质瘤组） 患者的标本 70 例， 非脑胶质瘤脑组织 10 例作为对照组。应用免疫组织化学 SP 法检测 2 组 OPN、 caspase-3 及 mt-P53 蛋白的阳性表达率。蛋白免疫印迹法检测脑胶质瘤中 OPN、 caspase-3 和 mt-P53 蛋白表达与脑胶质瘤级别的关系。Spearman 等级相关分析 OPN、 caspase-3 和 mt- P53 表达阳性的关系。结果 脑胶质瘤组 OPN、mt-P53 阳性表达率 （64.29%和 60%） 均高于对照组 （无阳性表达）， caspase-3 阳性率低于对照组 （47.14% vs. 90%）， 差异有统计学意义 （均 P＜0.05）。OPN、 caspase-3 和 mt-P53 在脑胶质瘤不同性别、 年龄、 肿瘤大小及肿瘤部位组间的阳性表达率差异无统计学意义 （P > 0.05）。胶质瘤分级越高， OPN和 mt-P53 的阳性表达率越高， 而 caspase-3 的阳性表达率越低 （均 P＜0.001）。随着脑胶质瘤级别的增加， OPN 和 mt- P53蛋白表达水平上调， 而caspase-3的蛋白表达水平下降。OPN与caspase-3阳性表达呈负相关， 与mt-P53表达呈正相关 （rs分别为-0.720和0.722， P＜0.05）， caspase-3和mt-P53表达呈负相关 （rs=-0.556， P＜0.05）。结论 胶质瘤分级越高， OPN和mt-P53的阳性表达率越高， 而caspase-3的阳性表达率越低； 三者与胶质瘤发生、 发展均密切相关， 联合检测有助于对胶质瘤生物学行为的判断。

关键词: 神经胶质瘤, 骨桥蛋白质, 半胱氨酸天冬氨酸蛋白酶3, 基因, p53

Abstract: Abstract: Objective To study the expressions of osteopontin (OPN)， caspase-3 and mt-P53 proteins, and their relationship in gliomas. Methods Seventy gliomas specimens of patients (glioma group) were selected, and 10 samples of non-glioma brain tissue were used as control group. The SP method was used to detect the positive rates of protein expressions of OPN, caspase- 3 and mt-P53 between two groups. The relationship between protein expressions of OPN, caspase-3 and mt-P53 in gliomas and grade of gliomas were detected by Western blot assay. Spearman rank correlation was compared between the positive expression of OPN, caspase-3 and rate mt-P53. Results The positive expression rates of OPN and mt-P53 were significantly higher in glioma group (64.29% and 60%) than those of control group (no positive expression), but the positive expression rate of caspase- 3 was significantly lower than that of control group (47.14% vs. 90%, P＜0.05). There were no significant differences in OPN, caspase-3 and mt-P53 expressions between different gender, age, tumor size and tumor position (P > 0.05). The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53 (P < 0.001), and the lower the positive expression rate of caspase-3 (P < 0.001). With the increased level of glioma grade, OPN and mt-P53 protein levels were increased, but caspase- 3 protein expression level was decreased. There was a negatively correlation between OPN and the positive expression of caspase- 3, but there was a positive correlation between OPN and the expression of mt-P53 (rs = -0.720 and 0.722, P < 0.05). There was a negative correlation between caspase-3 and mt-P53 expressions (rs = -0.556, P < 0.05). Conclusion The higher the WHO classification, the higher the positive expression rates of OPN and mt-P53, while the lower the positive expression rate of caspase- 3. The study reveals that OPN, caspase-3 and mt-P53 expressions are associated with the occurrence and the progress of gliomas. The combined detection of them can contribute to the judgment of biological behavior of gliomas.

Key words: glioma, osteopontin, caspase 3, genes, p53