天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 846-848.doi: 10.11958/20150266

• 实验研究 • 上一篇    下一篇

抑郁症大鼠海马-杏仁核pMAP-2表达改变及人参皂苷Rb1的干预作用

刘继刚 ， 袁杨 ， 臧蔚 ， 刘昊   

  1. 1遵化市人民医院口腔科（邮编 064200）；2华北理工大学附属医院神经内科
  • 收稿日期:2015-10-28 修回日期:2016-03-24 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 刘昊 E-mail:liuhao938@163.com
  • 作者简介:刘继刚（1962）， 男， 主治医师， 主要从事应激性疾病发生机制研究
  • 基金资助:
    国家自然科学基金资助项目（81201048）

The change of phosphorylated MAP-2 in hippocampus/amygdala and the influence of ginsenoside Rb1 on it in depressive rat model

LIU Jigang, YUAN Yang2 ZANG Yu, LIU Hao   

  1. 1 Department of Stomatology, Zunhua Peolple’ s Hospital, Zunhua 064200; 2 Department of Neurology, Affiliated Hospital of North China University of Science and Technology
  • Received:2015-10-28 Revised:2016-03-24 Published:2016-07-15 Online:2016-07-15
  • Contact: LIU Hao E-mail:liuhao938@163.com

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摘要： 目的 研究人参皂苷Rb1对抑郁大鼠海马-杏仁核组织微管相关蛋白-2（MAP-2）磷酸化的影响，探讨人参皂苷Rb1抗抑郁作用的可能机制。方法 选用30只成年Wistar大鼠，随机分为对照组、模型组和治疗组。采用慢性不可预性温和应激结合孤养方法建立大鼠抑郁症模型，治疗组同时给予人参皂苷Rb1。采用Western blot方法检测海马、杏仁核组织MAP-2及pMAP-2表达，Real-time PCR检测mRNA表达。结果 模型组大鼠海马和杏仁核组织MAP-2磷酸化程度和mRNA表达水平明显低于对照组（P < 0.05），治疗组表达明显升高，但仍低于对照组（P < 0.05）。结论 人参皂苷Rb1抗抑郁作用可能与影响海马、杏仁核MAP-2磷酸化有关

关键词: 人参皂苷Rb1, 抑郁症, 海马, 杏仁核, 微管相关蛋白

Abstract: Objectvie To observe the effcet of ginsenoside Rb1 on phosphorylation of microtubule-associated protein 2 (pMAP-2) in hippocampus and amygdala of depressive rats. Methods Thirty male Wistar rats were randomly divided into control group, model group and treatment group. The depression animal model was produced by giving the rats chronic unpredicted mild stress. The of pMAP-2 protein and mRNA were detected by western blot and real-time PCR respectively. Results The of pMAP-2 protein and mRNA of model group were lower than that of control group, while the index was higher after treatment with bupleurum liver-coursing powder (P<0.05). Conclusion Effect of ginsenoside Rb1 antidepressant may induced by affect the phosphorylation of MAP-2 in hippocampus.

Key words: ginsenoside Rb1, depression, hippocampus, amygdala, microtubule-associated protein