天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (3): 249-253.doi: 10.11958/20170049

• 实验研究 • 上一篇    下一篇

金芪降糖片对糖尿病大鼠肾脏 IL-17 和 IL-23表达的影响

吕元军 1， 李长平 2， 谭效锋 1， 李金娥 1， 崔壮 2△   

  1. 1 天津市天津医院普通内科 （邮编 300211）； 2 天津医科大学公共卫生学院
  • 收稿日期:2017-01-10 修回日期:2017-01-24 出版日期:2017-03-15 发布日期:2017-03-21
  • 通讯作者: 崔壮 E-mail:lyj22337@163.com
  • 基金资助:
    diabetes mellitus|diabetic nephropathies|interleukin-17|interleukin-23|Jinqi Jiangtang tablets|Th17

The effect of Jinqi Jiangtang tablet on expressions of IL-17 and IL-23 in kidney of diabetic rats

LYU Yuan-jun1, LI Chang-ping2, TAN Xiao-feng1, LI Jin-e1, CUI Zhuang2△   

  1. 1 Division of General Internal Medicine, Tianjin Hospital, Tianjin 300211, China;2 School of Public Health, Tianjin Medical University
  • Received:2017-01-10 Revised:2017-01-24 Published:2017-03-15 Online:2017-03-21
  • Contact: CUI Zhuang E-mail:lyj22337@163.com

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摘要： 摘要： 目的 探讨金芪降糖片对糖尿病大鼠辅助性 T 细胞（Th） 17 以及肾脏白细胞介素（IL） -17 和 IL-23 表达的影响。方法 雄性 SD 大鼠 45 只， 随机分为正常对照组（NC 组， 15 只）和造模组（30 只）， 造模组通过一次性尾静脉注射链脲佐菌素 （STZ， 45 mg/kg） 诱导建立糖尿病模型， 造模成功的 28 只大鼠随机分为糖尿病组 （DM 组） 和金芪降糖片治疗组 （金芪组， 2.1 g·kg-1·d-1）， 各 14 只。金芪组给予每日 1 次金芪降糖片溶液灌胃 18 周， NC 组和 DM 组给予等体积生理盐水灌胃。18 周末取材。流式细胞术测定外周血 Th17 表达频率； 双抗体两步夹心酶联免疫吸附法（ELISA） 测定血清 IL-17 和 IL-23 水平； 电镜观察各组大鼠肾小球超微结构改变； 免疫组化法测定肾脏 IL-17 和 IL-23 的表达水平。结果 （1） DM 组和金芪组大鼠外周血 Th17/CD4+细胞比例均较 NC 组显著上升， 金芪组大鼠外周血Th17/CD4+细胞比例较 DM 组降低。（2） DM 组和金芪组大鼠血清 IL-17、 IL-23 水平均较 NC 组显著上升， 金芪组 IL-17、 IL-23 水平较 DM 组降低。（3） 电镜下， DM 组大鼠肾小球毛细血管基底膜增厚， 部分足细胞突起融合， 系膜细胞增生； 金芪组大鼠肾小球病变较 DM 组减轻。（4） DM 组和金芪组大鼠肾脏 IL-17、 IL-23 表达均较 NC 组显著上升， 金芪组 IL-17、 IL-23 表达均较 DM 组降低。结论 Th17 活化、 肾脏 IL-17 和 IL-23 表达增多可能在糖尿病肾病发病过程中起重要作用。金芪降糖片可能通过抑制 Th17 活化和减少肾脏 IL-17 和 IL-23 的表达来改善糖尿病肾病。

关键词: 糖尿病, 糖尿病肾病, 白细胞介素 17, 白细胞介素 23, 金芪降糖片, 辅助性 T 细胞 17

Abstract: Abstract: Objective To investigate the effect of Jinqi Jiangtang tablet on the activation of T helper type 17 (Th17)and the expressions of interleukin (IL)-17 and IL-23 in kidney of diabetic rats. Methods A total of 45 male SD rats were randomly divided into normal control group (NC, n=15) and experimental group (n=30). Diabetes was induced by tail vein injection with streptozotocin (STZ, 45 mg/kg). The well-established 28 diabetic model rats were then randomly divided into diabetes group (DM, n=14) and Jinqi Jiangtang tablet administration group (Jinqi, n=14). The rats in Jinqi group were given Jinqi Jiangtang tablet solution by gavage at a single dose of 2.1 g·kg-1·d-1 for 18 weeks, while NC group and DM group were given 0.9% NaCl in the same way. All rats were sacrificed after 18 weeks. The circulating Th17 frequencies were assessed using flow cytometry. Serum IL- 17 and IL- 23 levels were measured by enzyme- linked immunosorbent assay. The pathological changes in kidney were studied by electron microscope. The expressions of IL- 17 and IL-23 in kidney were detected using immunohistochemistry. Results (1) Compared with the group NC, the circulating Th17 frequencies were significantly increased in group DM and group Jinqi. The circulating Th17 frequencies were significantly lower in group Jinqi than those in group DM. (2) Compared with the group NC, the serum IL-17 and IL-23 levels were significantly increased in group DM and group Jinqi. The serum IL-17 and IL-23 levels were significantly lower in group Jinqi than those in group DM. (3) In group DM, irregular thickening of glomerular basement membrane, fusion of epithelial cell foot processes and mesangial expansion were observed by electron microscope. The above-mentioned pathological changes were improved in the group Jinqi. (4) Compared with the group NC, the expressions of IL-17 and IL-23 in the renal cortex were significantly increased in group DM and group Jinqi, and those were significantly lower in group Jinqi than those in group DM.Conclusion The activation of Th17 and the increased expressions of IL-17 and IL-23 in kidney play a potential role in diabetic nephropathy. Jinqi Jiangtang tablet can improve diabetic nephropathy through inhibiting the activating Th17 and decreasing the expression of IL-17 and IL-23 in kidney.

Key words: diabetes mellitus, diabetic nephropathies, interleukin-17, interleukin-23, Jinqi Jiangtang tablets, Th17