天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (3): 280-283.doi: 10.11958/20171380

• 临床研究 • 上一篇    下一篇

CXCL10与CXCL12基因多态性与肺结核易感性的关系

邢志伟，高艳军，张建武，张珣，孙红梅   

  1. 1河北省胸科医院输血科（邮编050041），2检验科
  • 收稿日期:2017-12-04 修回日期:2018-01-19 出版日期:2018-03-15 发布日期:2018-03-23
  • 通讯作者: 张建武 E-mail:15633035239@163.com
  • 基金资助:
    河北省医学科学研究重点课题计划（20150601）

Association of gene polymorphism of CXCL10 and CXCL12 with tuberculosis susceptibility

XING Zhi-wei,GAO Yan-jun,ZHANG Jian-wu,ZHANG Xun,SUN Hong-mei   

  1. 1 Department of Blood Transfusion, 2 Department of Laboratory, Hebei Chest Hospital, Shijiazhuang 050041, China
  • Received:2017-12-04 Revised:2018-01-19 Published:2018-03-15 Online:2018-03-23

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摘要： 目的 探讨趋化因子 CXCL10-135G/A 和 CXCL12-801G/A 的基因多态性与肺结核病易感性之间的关系。方法 应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对 102 例结核病患者（病例组）和 115 例健康对照者（对照组）的 CXCL10-135G/A 和 CXCL12-801G/A 多态性进行基因分型，分析这两个位点多态性与结核病发生是否相关。结果 病例组和对照组 CXCL10-135G/A 与 CXCL12-801G/A 基因型分布符合 Hardy-Weinberg 平衡定律。CXCL10-135G/A 多态性基因型及等位基因的分布频率在病例组及对照组中差异均有统计学意义（均 P＜ 0.05），病例组 G 等位基因的分布频率高于对照组，A 等位基因的分布频率低于对照组。CXCL12-801G/A 多态性基因型及等位基因的分布频率在病例组及对照组中差异均无统计学意义（均 P＞0.05）。结论 趋化因子 CXCL10-135G/A 基因多态性与肺结核易感性有关，而 CXCL12-801G/A 基因多态性与肺结核感染可能无关。

关键词: 趋化因子 CXCL10, 趋化因子 CXCL12, 多态性, 单核苷酸, 结核, 肺, 疾病遗传易感性

Abstract: Objective To explore the relationship of chemokines CXCL10-135G / A and CXCL12 - 801G / A gene polymorphisms with susceptibility to tuberculosis. Methods CXCL10-135G/A and CXCL12 -801G/A polymorphisms of 102 tuberculosis patients (case group) and 115 healthy controls (control group) were genotyped by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP), and the relationship between the two polymorphisms and susceptibility to tuberculosis were analyzed. Results The genotype analysis of CXCL10-135G/A and CXCL12 -801G/A was in accord with the law of Hardy-Weinberg equilibrium in the case group and the control group. The differences of genotype and allele distribution frequency of CXCL10-135G/A were statistically significant between the case group and the control group (all P＜0.05). The frequency of G allele distribution was higher in the case group than that in the control group,and the frequency of A allele distribution was lower than that in the control group. There were no significant differences in genotype and allele distribution frequency of CXCL12-801G/A polymorphism between the case group and the control group(all P＞0.05). Conclusion Chemokine CXCL10-135G/A gene polymorphism is associated with susceptibility to pulmonary tuberculosis, and CXCL12-801G/A gene polymorphism may not be associated with tuberculosis infection.

Key words: 趋化因子 CXCL10, 趋化因子 CXCL12, 多态性, 单核苷酸, 结核, 肺, 疾病遗传易感性