天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (8): 847-851.doi: 10.11958/20180136

• 实验研究 • 上一篇    下一篇

银杏叶提取物对二甲苯中毒性肝损伤防治机制的研究

米红梅1 ， 张娜2 ， 刘宁1   

  1. 1河北省衡水市第四人民医院消化科 （邮编053000）； 2河北省衡水市妇幼保健院儿科
  • 收稿日期:2018-01-24 修回日期:2018-04-21 出版日期:2018-08-15 发布日期:2018-08-23
  • 通讯作者: 米红梅 E-mail:hongmei_20062008@163.com
  • 基金资助:
    衡水市科技计划项目

Preventive and therapeutic mechanism of ginkgo biloba extract on liver injury poisoned by xylene

MI Hong-mei 1 , ZHANG Na2 , LIU Ning1   

  1. 1 Department of Gastroenterology, The No.4 People’ s Hospital of Hengshui, Hebei 053000, China; 2 Department of Pediatrics, The Maternal and Child Care Hospital of Hengshui
  • Received:2018-01-24 Revised:2018-04-21 Published:2018-08-15 Online:2018-08-23

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摘要：  目的 探讨银杏叶提取物 （GBE） 对二甲苯中毒小鼠肝组织炎性因子表达的影响及作用机制。方法 将清洁级健康昆明种小鼠30只随机均分为正常对照 （Control） 组、 模型 （Model） 组、 银杏叶提取物干预 （GBE） 组。3组均自由进食， Model组和GBE组均通过吸入浓度为110～130 mg/m3 （采集箱内空气样本， 用气相色谱直接进样法测得） 气体二甲苯， Control组小鼠吸入空气。GBE组腹腔注射50 mg/ （kg·d） 的银杏叶提取物注射液； Control组和Model组均腹腔注射等量生理盐水。8周后处死动物， 留取血清、 肝组织备用。检测血清丙氨酸转氨酶 （ALT）、 天冬氨酸转氨酶（AST）、 总胆红素 （TBIL）、 碱性磷酸酶 （ALP）、 γ-谷氨酰转移酶 （GGT） 水平， HE染色观察肝组织病理改变， 采用RT- PCR和Western blot检测肝组织核转录因子 （NF） -κB、 肿瘤坏死因子 （TNF） -α mRNA及蛋白表达。结果 与Control 组比较， Model组肝组织损伤严重， 血清生化指标 （ALT、 AST、 TBIL、 ALP、 GGT） 明显升高， NF-κB、 TNF-α mRNA和蛋白表达均明显升高 （均P＜0.05）； 与Model组比较， GBE组小鼠肝组织损伤程度减轻， 血清生化指标 （ALT、 AST、 TBIL）降低， NF-κB、 TNF-α mRNA和蛋白表达量下降 （均P＜0.05）。结论 银杏叶提取物通过抑制NF-κB、 TNF-α炎性因子的表达， 从而减轻二甲苯中毒小鼠肝组织炎症反应。

关键词: 二甲苯类, NF-κB, 肿瘤坏死因子α, 银杏叶提取物, 肝损伤

Abstract:  Objective To investigate the potential effect and molecular mechanism of ginkgo biloba extract (GBE) on the expression of inflammatory factors in liver tissue of mice poisoned by xylene. Methods A total of 30 clean grade healthy Kunming mice were randomly divided into normal control group, model group and ginkgo biloba extract intervention group (GBE group). Mice of three groups were granted free access to food and water. The concentration of xylene inhalation was 110-130 mg/m3 (the air samples were collected in the box, and the gas chromatogram was measured by direct injection method) in model group and GBE group. The mice in control group inhaled air. GBE group was intraperitoneally injected with 50 mg/(kg·d) of GBE. Control and model groups were intraperitoneal injected with same amount of normal saline. After 8 weeks, mice were sacrificed, and serum and liver tissues were retained. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP) and glutamyl transpeptidase (GGT) were measured. Haematoxylin and Eosin staining was used to observe pathological changes of liver tissue. RT-PCR and Western blot assay were used to detect the expression levels of nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α) mRNA and protein in liver tissue. Results Compared with the control group, the liver tissue was damage seriously in the model group. The blood biochemical indexes (ALT, AST, TBIL, ALP and GGT) were significantly increased (P＜0.05), and the NF-κB, TNF-α mRNA and protein were significantly increased in the model group (P＜0.05). Compared with the model group, the liver tissue damage was reduced, the blood biochemical indexes (ALT, AST, TBIL, ALP and GGT) were significantly decreased (P＜0.05) and the NF-κB, TNF-α mRNA and protein were significantly down-regulated in the GBE group (P＜0.05). Conclusion Ginkgo biloba extract can reduce hepatic inflammatory response of mice poisoned by xylene through inhibiting the expression of NF-κB and TNF-α inflammatory factors.

Key words: xylenes, NF-kappa B, tumor necrosis factor-alpha, ginkgo biloba extract, liver injury