天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (12): 1291-1294.doi: 10.11958/20181203

• 实验研究 • 上一篇    下一篇

原花青素对环磷酰胺致免疫抑制小鼠免疫功能的影响

孔妮，刘超，杨方浩，张腾元，王宝堃，高慧婕   

  1. 1济宁医学院药学院（邮编276826）；2生物科学学院
  • 收稿日期:2018-08-13 修回日期:2018-10-19 出版日期:2018-12-15 发布日期:2019-01-24
  • 通讯作者: 高慧婕 E-mail:mianyigao@163.com

Effects of procyanidins on immune function in cyclophosphamide - induced immunosuppression mice

KONG Ni,LIU Chao,YANG Fang-hao,ZHANG Teng-yuan,WANG Bao-kun,GAO Hui-jie   

  1. 1 Department of Pharmacy, 2 School of Biological Science, Jining Medical University, Rizhao 276826, China
  • Received:2018-08-13 Revised:2018-10-19 Published:2018-12-15 Online:2019-01-24

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摘要： 目的 探究原花青素（PC）对环磷酰胺（Cy）致免疫抑制小鼠的免疫调节作用及其对白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）表达的影响。方法 选用40只ICR小鼠，随机均分为正常对照组、Cy模型组、PC高剂量组（100 mg/kg）和PC低剂量组（80 mg/kg）。灌胃给药，7 d后测定各组小鼠胸腺（脾脏）指数、血清溶血素水平、迟发型超敏反应性；酶联免疫吸附测定（ELISA）检测外周血IL-10和TNF-α的表达；RT-qPCR测定小鼠脾脏TNF-α和IL-10 mRNA的表达。结果 PC能够提高小鼠胸腺、脾脏指数，一定程度恢复免疫抑制小鼠的迟发型超敏反应能力，提高血清溶血素含量；同时PC也可降低外周血IL-10的表达，升高TNF-α的表达；降低脾细胞IL-10 mRNA的水平，升高TNF-α mRNA的水平。结论 原花青素可以提升机体免疫功能，其调节机制可能是通过对细胞因子TNF-α和IL-10的调节来完成的。

关键词: 花青苷类, 环磷酰胺, 白细胞介素10, 肿瘤坏死因子α, 小鼠, 近交ICR

Abstract: Objective To investigate the immunoregulation effect of Proantho Cyanidins (PC) and its effect on the expressions of TNF-α and IL-10 in immunosuppression mice. Methods Forty institute of cancer research (ICR) mice were randomly divided into four groups: control group, cyclophosphamide (Cy) model group, PC high-dose (100 mg/kg) group and PC low-dose (80 mg/kg) group. After 7 days, the thymus and spleen index, the content of serum hemolysin and the delayed type hypersensitivity (DTH) were analyzed. The expressions of TNF - α and IL-10 in peripheral blood were detected by ELISA. RT- qPCR was used to detect the changes of TNF-α and IL-10 mRNA expressions in mouse spleen cells. Results The application of procyanidins increased the thoracic spleen index, the serum hemolysin level and the level of delayed type hypersensitivity in mice. The level of IL-10 was decreased, and the level of TNF-α was increased in procyanidin group. The mRNA expression of TNF-α in spleen cells was increased, and the expression of IL-10 mRNA was decreased. Conclusion Procyanidins could improve the immune function, which is likely to be accomplished through the regulation of the cytokines TNF-α and IL-10.

Key words: anthocyanins, cyclophosphamide, interleukin-10, tumor necrosis factor-alpha, mice, inbred ICR